ESTABLISHMENT OF RT-PCR FOR DETECTION OF AVIAN INFLUENZA VIRUS (H9N2) IN FIELD CASES COMPARED TO VIRUS ISOLATION METHOD

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TAJMANESH SHOKOUFEH; TOROGHI S.; MOMAYEZ R.; POURBAKHSH S.A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARCHIVES OF RAZI INSTITUTE Year:2006 | Volume:61 | Issue:2 Page(s): 111-115

Download Full-Text

Persian Verion

View:

1,806

Download:

Cites:

Information Journal Paper

Title

ESTABLISHMENT OF RT-PCR FOR DETECTION OF AVIAN INFLUENZA VIRUS (H9N2) IN FIELD CASES COMPARED TO VIRUS ISOLATION METHOD

Author(s)

TAJMANESH SHOKOUFEH | TOROGHI S. | MOMAYEZ R. | POURBAKHSH S.A. | Issue Writer Certificate

Keywords

AVIAN INFLUENZA VIRUSQ4

H9N2Q4

RT-PCRQ4

VIRUS ISOLATIONQ4

Abstract

Avian influenza (AI) is one of the most economical viral diseases in Iranian poultry industry because of increased mortality and declines in egg production in affected chicken flocks. VIRUS ISOLATION in embryonated SPF chicken eggs, a time consuming method, is the most common diagnostic test. In this study, VIRUS ISOLATION and RT-PCR for detection of AVIAN INFLUENZA VIRUS were evaluated by use of trachea samples collected from ten clinically affected broiler flocks. Ten percent trachea tissue suspension was inoculated to 10-day-old embryonated SPF chicken eggs. AVIAN INFLUENZA VIRUS, H9N2 subtype, was confirmed in dead embryos by hemagglutination inhibition test. Simultaneously, total RNA was extracted from the trachea tissues and RT-PCR was optimized for amplification of a 488 bp DNA fragment of H9N2 subtype hemagglutinin gene. In seven of the ten flocks both the RT- PCR and VIRUS ISOLATION methods detected the virus. In two flocks AI virus was not detected by either method, while in a flock AI virus was identified only by RT-PCR. Newcastle disease virus was isolated in one of the flocks in which was negative by both the methods. The sensitivity and specificity rate by RT-PCR in comparison with VIRUS ISOLATION as gold standard test was coordinately 100% and 66%, respectively. The detection of AI by RT- PCR showed the best correlation with the VIRUS ISOLATION method even more sensitive than it. It is suggested that in surveillance centers where early detection of AI virus is goal, RT-PCR is a reliable method for rapid laboratory diagnosis of influenza in dead chickens because of its speed, sensitivity and specificity.

Cites

EVALUATION OF H9N2 AVIAN INFLUENZA VIRUS DISSEMINATION IN VARIOUS ORGANS OF EXPERIMENTALLY INFECTED BROILER CHICKENS USING RT-PCR

References

No record.

Cite

APA: Copy

TAJMANESH, SHOKOUFEH, TOROGHI, S., MOMAYEZ, R., & POURBAKHSH, S.A.. (2006). ESTABLISHMENT OF RT-PCR FOR DETECTION OF AVIAN INFLUENZA VIRUS (H9N2) IN FIELD CASES COMPARED TO VIRUS ISOLATION METHOD. ARCHIVES OF RAZI INSTITUTE, 61(2), 111-115. SID. https://sid.ir/paper/120035/en

Vancouver: Copy

TAJMANESH SHOKOUFEH, TOROGHI S., MOMAYEZ R., POURBAKHSH S.A.. ESTABLISHMENT OF RT-PCR FOR DETECTION OF AVIAN INFLUENZA VIRUS (H9N2) IN FIELD CASES COMPARED TO VIRUS ISOLATION METHOD. ARCHIVES OF RAZI INSTITUTE[Internet]. 2006;61(2):111-115. Available from: https://sid.ir/paper/120035/en

IEEE: Copy

SHOKOUFEH TAJMANESH, S. TOROGHI, R. MOMAYEZ, and S.A. POURBAKHSH, “ESTABLISHMENT OF RT-PCR FOR DETECTION OF AVIAN INFLUENZA VIRUS (H9N2) IN FIELD CASES COMPARED TO VIRUS ISOLATION METHOD,” ARCHIVES OF RAZI INSTITUTE, vol. 61, no. 2, pp. 111–115, 2006, [Online]. Available: https://sid.ir/paper/120035/en

Related Journal Papers