Correlation between Two RT-PCR Primers in Detection of Avian Influenza Viruses (H9N2)

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Mohammad Bagher Pour K.; POURTAGHI H.; HOSSEINI H.

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Journal: Veterinary Research & Biological Products Year:2020 | Volume:32 | Issue:4 (125) Page(s): 38-43

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Title

Correlation between Two RT-PCR Primers in Detection of Avian Influenza Viruses (H9N2)

Author(s)

Mohammad Bagher Pour K. | POURTAGHI H. | HOSSEINI H. | Issue Writer Certificate

Keywords

Avian Influenza VirusQ2

H9N2Q2

CorrelationQ2

Abstract

The goal of current study was comparison of two RT-PCR primers in detection of H9N2 influenza viruses. We compare Correlation between two methods to determine which method in serial dilution can detect more samples. First, EID50 of an Avian Influenza Virus was calculated by Reed-Muench method. The titer of virus was 10 EID50/ml. Then, we prepare two fold serial dilution of virus. Then, genome was extracted by RNA Pure kit (Cinna-Gene, Iran). Two separate one step RT-PCR methods were used to detection of Avian Influenza Virus (Wu et al. 2008 and Spackman et al. 2002). Target gene of both methods was matrix (M) gene. Product size that produced by both methods were 131 bp and 100 bp respectively. To ensure about specifity of two RT-PCR primers, we used H120 vaccine and B1 vaccine as negative control. Both methods were specific to Avian Influenza Virus and none of them did not produce any amplicons when used other respiratory disease viruses as template. The Wu et al, 2008 primers was able to detect Avian Influenza Virus to 1: 4096 and 168 copy number/ml but, Spackman et al, 2000 primers was only able to detect 1: 2048 and 336 copy number/ml. So, the Wu et al, 2008 primers was more sensitive method in detection of Avian Influenza Viruses.

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APA: Copy

POURTAGHI, H., Mohammad Bagher Pour, K., & HOSSEINI, H.. (2020). Correlation between Two RT-PCR Primers in Detection of Avian Influenza Viruses (H9N2). VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 32(4 (125) ), 38-43. SID. https://sid.ir/paper/397560/en

Vancouver: Copy

POURTAGHI H., Mohammad Bagher Pour K., HOSSEINI H.. Correlation between Two RT-PCR Primers in Detection of Avian Influenza Viruses (H9N2). VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)[Internet]. 2020;32(4 (125) ):38-43. Available from: https://sid.ir/paper/397560/en

IEEE: Copy

H. POURTAGHI, K. Mohammad Bagher Pour, and H. HOSSEINI, “Correlation between Two RT-PCR Primers in Detection of Avian Influenza Viruses (H9N2),” VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), vol. 32, no. 4 (125) , pp. 38–43, 2020, [Online]. Available: https://sid.ir/paper/397560/en

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