CLONING AND SEQUENCING OF TOXOPLASMA GONDII MAJOR SURFACE ANTIGEN (SAGL) GENE

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SOLHJOO K.; GHAFFARIFAR FATEMEH; DALIMI A.; SHARIFI Z.

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Journal: ARCHIVES OF RAZI INSTITUTE Year:2006 | Volume:61 | Issue:2 Page(s): 73-79

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Information Journal Paper

Title

CLONING AND SEQUENCING OF TOXOPLASMA GONDII MAJOR SURFACE ANTIGEN (SAGL) GENE

Author(s)

SOLHJOO K. | GHAFFARIFAR FATEMEH | DALIMI A. | SHARIFI Z. | Issue Writer Certificate

Keywords

CLONINGQ4

SEQUENCINGQ4

TOXOPLASMA GONDIIQ4

SAGLQ4

P30Q4

Abstract

Genetic typing methods of T. gondii strains have been extensively perfected in recent years. From a technical point of view, many tools usable for genetic studied on single-copy loci have been used: RFLP, PCR-RFLP, SEQUENCING, RAPD-PCR and isoenzyme analysis. We described the CLONING and sequence analysis of the gene which encodes the major surface antigen (SAG1 or P30) of T. gondii. SAG1 is the immunodominant antigen of TOXOPLASMA GONDII tachyzoites being considered as the most promising molecule for a recombinant vaccine or such as DNA vaccine against toxoplasmosis. In the present work, first, genomic DNA of TOXOPLASMA GONDII was extracted and used for amplifying of SAG 1 gene as a template. Then PCR product was cloned into pTZ57R/T vector and plasmid containing SAGL gene (pTSAG1) was extracted from transformed bacteria and SAGL gene cloned into pTZ57R/T was sequenced.Results showed that the P30 gene contains no introns and can extract it from genomic DNA of tachyzoite stage. Results showed also that SAG 1 gene is cloned in pTZ57R/T plasmid, forming pT-SAGl recombinant plasmid and E. coli TGl strain is the best host for pT-SAGl transformation. Sequence analysis of SAG 1 gene cloned into pTZ57R/T vector showed that SAG 1 gene sequence from a high virulent strain of T. gondii (Known as RH strain) has 100% sequence identity with P-Br strain, P strain and C strain and high homology of 98% with RH strain and ZSl strain.

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APA: Copy

SOLHJOO, K., GHAFFARIFAR, FATEMEH, DALIMI, A., & SHARIFI, Z.. (2006). CLONING AND SEQUENCING OF TOXOPLASMA GONDII MAJOR SURFACE ANTIGEN (SAGL) GENE. ARCHIVES OF RAZI INSTITUTE, 61(2), 73-79. SID. https://sid.ir/paper/120036/en

Vancouver: Copy

SOLHJOO K., GHAFFARIFAR FATEMEH, DALIMI A., SHARIFI Z.. CLONING AND SEQUENCING OF TOXOPLASMA GONDII MAJOR SURFACE ANTIGEN (SAGL) GENE. ARCHIVES OF RAZI INSTITUTE[Internet]. 2006;61(2):73-79. Available from: https://sid.ir/paper/120036/en

IEEE: Copy

K. SOLHJOO, FATEMEH GHAFFARIFAR, A. DALIMI, and Z. SHARIFI, “CLONING AND SEQUENCING OF TOXOPLASMA GONDII MAJOR SURFACE ANTIGEN (SAGL) GENE,” ARCHIVES OF RAZI INSTITUTE, vol. 61, no. 2, pp. 73–79, 2006, [Online]. Available: https://sid.ir/paper/120036/en

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