COMPARISON OF SEVERAL METHODS FOR DETECTION AND QUANTIFICATION OF ß- EXOTOXIN IN COMMERCIAL BACILLUS THURINGIENSIS PRODUCTS

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MARZBAN R.; TAJBAKHSH M.

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Journal: Applied Entomology and Phytopathology Year:2004 | Volume:71 | Issue:2 Page(s): 141-149

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Title

COMPARISON OF SEVERAL METHODS FOR DETECTION AND QUANTIFICATION OF ß- EXOTOXIN IN COMMERCIAL BACILLUS THURINGIENSIS PRODUCTS

Author(s)

MARZBAN R. | TAJBAKHSH M. | Issue Writer Certificate

Keywords

BACILLUS THURINGIENSIS SUBSP. AIZAWAIQ4

BETA-EXOTOXINQ4

HPLCQ2

BIOASSAYQ2

BILLQ4

Abstract

The ß -exotoxin (also called thuingiensin and thermo stable toxin) is toxic to human and other animals. Because of vertebrate toxicity, most commercial preparation of B. thuringiensis is composed of subspecies or isolates that do not produce ß -exotoxin. As a condition for registration for pesticide use on food in USA, B. thuringiensis active ingredients must be tested to show the abscense of ß -exotoxin. In this study screening methods for the presence of ß-exotoxin in a product of B. thuringiensis subsp. aizawai (Bill) were based on laboratory BIOASSAY against lepidopteran larvae and High Performance Liquid Chromatography (HPLC). The BIOASSAY and HPLC assay results indicated that the ß -exotoxin was presence in the product of B. thuringiensis subsp. aizawai (Bill). BIOASSAY was showed to be more sensitive than High Performance Liquid Chromatography (HPLC). BIOASSAY can be used to detect and qualify B. thuringiensis ß -exotoxin and can be a useful tool for quality control.

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APA: Copy

MARZBAN, R., & TAJBAKHSH, M.. (2004). COMPARISON OF SEVERAL METHODS FOR DETECTION AND QUANTIFICATION OF ß- EXOTOXIN IN COMMERCIAL BACILLUS THURINGIENSIS PRODUCTS. APPLIED ENTOMOLOGY AND PHYTOPATHOLOGY, 71(2), 141-149. SID. https://sid.ir/paper/13027/en

Vancouver: Copy

MARZBAN R., TAJBAKHSH M.. COMPARISON OF SEVERAL METHODS FOR DETECTION AND QUANTIFICATION OF ß- EXOTOXIN IN COMMERCIAL BACILLUS THURINGIENSIS PRODUCTS. APPLIED ENTOMOLOGY AND PHYTOPATHOLOGY[Internet]. 2004;71(2):141-149. Available from: https://sid.ir/paper/13027/en

IEEE: Copy

R. MARZBAN, and M. TAJBAKHSH, “COMPARISON OF SEVERAL METHODS FOR DETECTION AND QUANTIFICATION OF ß- EXOTOXIN IN COMMERCIAL BACILLUS THURINGIENSIS PRODUCTS,” APPLIED ENTOMOLOGY AND PHYTOPATHOLOGY, vol. 71, no. 2, pp. 141–149, 2004, [Online]. Available: https://sid.ir/paper/13027/en

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