DETERMINATION OF Β-EXOTOXIN IN IRANIAN ISOLATES OF BACILLUS THURINGIENSIS

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IZADYAR S.; TALEBI JAHROMI KH.; ASKARI H.; REZAPANAH M.R.

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Journal: Journal of Entomological Society of Iran Year:2003 | Volume:23 | Issue:1 Page(s): 55-68

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Title

DETERMINATION OF Β-EXOTOXIN IN IRANIAN ISOLATES OF BACILLUS THURINGIENSIS

Author(s)

IZADYAR S. | TALEBI JAHROMI KH. | ASKARI H. | REZAPANAH M.R. | Issue Writer Certificate

Keywords

IRANIAN STRAINS OF BACILLUS THURINGIENSISQ4

Β-EXOTOXINQ4

HELICOVERPA ARMIGERAQ2

HPLCQ4

BIOASSAYQ3

Abstract

Twelve Iranian isolates of B. thuringiensis were evaluated for Β-EXOTOXIN using combination of BIOASSAY and HPLC methods. BIOASSAY results on 4 days old larvae of HELICOVERPA ARMIGERA showed the existence of a mortality factor, which can be β -exotoxin, in some autoclaved supernatant of isolates (with no spores and crystals). To verify these results, the β -exotoxin was measured in each isolate using HPLC method. The results indicated that the concentrated supernatant of isolates Krm23, Kn4, 3R and 4t contained β -exotoxin. Isolates Krm23 and 3R had the lowest (7.37 µg/ml) and highest (294.5 µg/ml) amounts of β -exotoxin, respectively. Comparison of BIOASSAY results with Β-EXOTOXIN analyzed by HPLC showed that measured quantities of β –exotoxin were confirmed by the mortality percentage of HELICOVERPA ARMIGERA.

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APA: Copy

IZADYAR, S., TALEBI JAHROMI, KH., ASKARI, H., & REZAPANAH, M.R.. (2003). DETERMINATION OF Β-EXOTOXIN IN IRANIAN ISOLATES OF BACILLUS THURINGIENSIS. JOURNAL OF ENTOMOLOGICAL SOCIETY OF IRAN (JESI), 23(1), 55-68. SID. https://sid.ir/paper/47805/en

Vancouver: Copy

IZADYAR S., TALEBI JAHROMI KH., ASKARI H., REZAPANAH M.R.. DETERMINATION OF Β-EXOTOXIN IN IRANIAN ISOLATES OF BACILLUS THURINGIENSIS. JOURNAL OF ENTOMOLOGICAL SOCIETY OF IRAN (JESI)[Internet]. 2003;23(1):55-68. Available from: https://sid.ir/paper/47805/en

IEEE: Copy

S. IZADYAR, KH. TALEBI JAHROMI, H. ASKARI, and M.R. REZAPANAH, “DETERMINATION OF Β-EXOTOXIN IN IRANIAN ISOLATES OF BACILLUS THURINGIENSIS,” JOURNAL OF ENTOMOLOGICAL SOCIETY OF IRAN (JESI), vol. 23, no. 1, pp. 55–68, 2003, [Online]. Available: https://sid.ir/paper/47805/en

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