In Vitro Evaluation of Equine Fibroblast-Like Synoviocytes Viability Treated with Doxycycline

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GHASEMI SAMANEH; Sardari Kamran; Mirshokraei Pezhman; HASSANPOUR HOSSEIN

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Journal: Iranian Journal of Veterinary Surgery Year:2017 | Volume:12 | Issue:1 (26) Page(s): 11-17

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Title

In Vitro Evaluation of Equine Fibroblast-Like Synoviocytes Viability Treated with Doxycycline

Author(s)

GHASEMI SAMANEH | Sardari Kamran | Mirshokraei Pezhman | HASSANPOUR HOSSEIN | Issue Writer Certificate

Keywords

DoxycyclineQ2

Equine fibroblast-like synoviocytesQ1

MTT assayQ1

Trypn blueQ1

ViabilityQ1

Abstract

Objective-The purpose of the present study was to investigate the Viability of Equine fibroblast-like synoviocytes (FLSs) treated with Doxycycline. Design-Experimental study. Animals-FLSs from metacarpophalangeal joints of six skeletally mature horses. Methods-FLSs were established from synovial fluids of healthy joints. The cells were treated with varoius concentrations (1, 5, 10, 50, 100, 150, 300, 400 μ g/ml) or without Doxycycline for 48-hour. Viability of FLSs was determined using MTT assay and the trypan blue dye exclusion method. Results-No significant differences were observed between Viability of FLSs cultures treated with Doxycycline until 150 μ g/ml and control group (P>0. 05). Doxycycline at 300 and 400 μ g/ml significantly decreased FLSs Viability (P<0. 05). FLSs Viability was 74. 28% and 59. 07% in 300 and 400 μ g/ml, respectively, when measured by the MTT assay. Also FLSs Viability at 300 and 400 μ g/ml of Doxycycline were 68. 10% and 43. 26%, respectively, with trypan blue exclusion method. Conclusion and Clinical Relevance-These findings demonstrated that Doxycycline was not toxic for equine FLSs at concentration ≤ 150 μ g/ml in vitro. Further studies are needed to investigate the safety, efficacy and detrimental effects of Doxycycline in equine joints.

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APA: Copy

GHASEMI, SAMANEH, Sardari, Kamran, Mirshokraei, Pezhman, & HASSANPOUR, HOSSEIN. (2017). In Vitro Evaluation of Equine Fibroblast-Like Synoviocytes Viability Treated with Doxycycline. IRANIAN JOURNAL OF VETERINARY SURGERY (IJVS), 12(1 (26) ), 11-17. SID. https://sid.ir/paper/135743/en

Vancouver: Copy

GHASEMI SAMANEH, Sardari Kamran, Mirshokraei Pezhman, HASSANPOUR HOSSEIN. In Vitro Evaluation of Equine Fibroblast-Like Synoviocytes Viability Treated with Doxycycline. IRANIAN JOURNAL OF VETERINARY SURGERY (IJVS)[Internet]. 2017;12(1 (26) ):11-17. Available from: https://sid.ir/paper/135743/en

IEEE: Copy

SAMANEH GHASEMI, Kamran Sardari, Pezhman Mirshokraei, and HOSSEIN HASSANPOUR, “In Vitro Evaluation of Equine Fibroblast-Like Synoviocytes Viability Treated with Doxycycline,” IRANIAN JOURNAL OF VETERINARY SURGERY (IJVS), vol. 12, no. 1 (26) , pp. 11–17, 2017, [Online]. Available: https://sid.ir/paper/135743/en

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