ASSESS THE PLURIPOTENCY OF CAPRINE UMBILICAL CORD WHARTON’S JELLY MESENCHYMAL CELLS BY RT-PCR ANALYSIS OF EARLY TRANSCRIPTION FACTOR NANOG

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BABAEI HOMAYOUN; MOSHREFI MOZHGAN; GOLCHIN MAHDI; NEMAT ELAHI MAHANI S.N.A.D.

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Journal: Iranian Journal of Veterinary Surgery Year:2008 | Volume:3 | Issue:2 (S.N. 7 ) Page(s): 57-65

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Title

ASSESS THE PLURIPOTENCY OF CAPRINE UMBILICAL CORD WHARTON’S JELLY MESENCHYMAL CELLS BY RT-PCR ANALYSIS OF EARLY TRANSCRIPTION FACTOR NANOG

Author(s)

BABAEI HOMAYOUN | MOSHREFI MOZHGAN | GOLCHIN MAHDI | NEMAT ELAHI MAHANI S.N.A.D. | Issue Writer Certificate

Keywords

WHARTON'S JELLYQ3

MESENCHYMAL CELLSQ3

ALKALINE PHOSPHATASEQ3

GROWTH KINETICQ2

NANOGQ3

Abstract

Objective: In the present study we investigated the isolation protocol, population doubling time (PDT) and the expression of a pluripotential gene by RT-PCR analysis of early transcription factor NANOG in caprine umbilical cord (CUC) WHARTON'S JELLY MESENCHYMAL CELLS (WJMCs).Design: Experimental in vitro study.Animals: Four mix breed goatProcedures: CUCs were collected from abattoir pregnant uteri and their Wharton's jellies (WJs) were cut into 2×2 mm2 segments for explanting. 8-10 segments were explanted into each 35 mm culture dish. WJ explants were removed 5 days after plating and the remaining adherent cells were cultured for another 5 days. Isolated cells were histochemically assayed for the presence of ALKALINE PHOSPHATASE (AP) activity. RT-PCR was used to assess the presence of NANOG mRNA. Besides, in this study the GROWTH KINETIC was evaluated for the isolated cells.Results: CUC isolated cells displayed spindle-form and small round-shape with high nucleus. Confluent cells formed colonies that indicated AP activity. Initial seeding concentration of 2×104 CUC-WJMCs resulted in shorter PDT compared to fetal fibroblasts (46.57 vs. 54.29 hours, respectively). Expression of NANOG was undetectable in 9th passage of CUC-WJMCs.Conclusion and Clinical Relevance: CUC contains an easily obtainable source of MESENCHYMAL CELLS which exhibit stem cells properties but probably these cells are not pluripotent.

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APA: Copy

BABAEI, HOMAYOUN, MOSHREFI, MOZHGAN, GOLCHIN, MAHDI, & NEMAT ELAHI MAHANI, S.N.A.D.. (2008). ASSESS THE PLURIPOTENCY OF CAPRINE UMBILICAL CORD WHARTON’S JELLY MESENCHYMAL CELLS BY RT-PCR ANALYSIS OF EARLY TRANSCRIPTION FACTOR NANOG. IRANIAN JOURNAL OF VETERINARY SURGERY (IJVS), 3(2 (S.N. 7 )), 57-65. SID. https://sid.ir/paper/135762/en

Vancouver: Copy

BABAEI HOMAYOUN, MOSHREFI MOZHGAN, GOLCHIN MAHDI, NEMAT ELAHI MAHANI S.N.A.D.. ASSESS THE PLURIPOTENCY OF CAPRINE UMBILICAL CORD WHARTON’S JELLY MESENCHYMAL CELLS BY RT-PCR ANALYSIS OF EARLY TRANSCRIPTION FACTOR NANOG. IRANIAN JOURNAL OF VETERINARY SURGERY (IJVS)[Internet]. 2008;3(2 (S.N. 7 )):57-65. Available from: https://sid.ir/paper/135762/en

IEEE: Copy

HOMAYOUN BABAEI, MOZHGAN MOSHREFI, MAHDI GOLCHIN, and S.N.A.D. NEMAT ELAHI MAHANI, “ASSESS THE PLURIPOTENCY OF CAPRINE UMBILICAL CORD WHARTON’S JELLY MESENCHYMAL CELLS BY RT-PCR ANALYSIS OF EARLY TRANSCRIPTION FACTOR NANOG,” IRANIAN JOURNAL OF VETERINARY SURGERY (IJVS), vol. 3, no. 2 (S.N. 7 ), pp. 57–65, 2008, [Online]. Available: https://sid.ir/paper/135762/en

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