THE COMPARISON OF CEL I ENDONUCLEASE GENE EXPRESSION IN SOME APIACEAE PLANTS USING SEMI-QUANTITATIVE RT-PCR

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ZOLALA J.; FARSI MOHAMMAD; BAHRAMI AHMAD REZA; MOGHADAM MATIN M.; GHABOULI M.

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Journal: Journal of Horticultural Science Year:2008 | Volume:22 | Issue:1 Page(s): 3-11

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Title

THE COMPARISON OF CEL I ENDONUCLEASE GENE EXPRESSION IN SOME APIACEAE PLANTS USING SEMI-QUANTITATIVE RT-PCR

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Keywords

SEMI-QUANTITATIVE RT-PCRQ3

CEL I ENZYMEQ3

DIFFERENTIAL GENE EXPRESSIONQ3

APIACEAEQ3

Abstract

The CEL I ENZYME, a technical glycoprotein associated with the S1 family of endonucleases, is the first eukaryotic nuclease known for cleaving DNA with high specificity at sites of base–substitution mismatch and DNA distortion. CEL I assay is a highly sensitive method for detecting both polymorphisms and disease-causing mutations in DNA molecules, with a wide range of applications to research and medical genetics. So far, this enzyme has been produced in native form by isolating it from celery tissues according to Yang et al., 2000, but it appears to be a complex and expensive method for obtain this valuable enzyme. In this research, with the aim of improving the efficiency of enzyme isolation, we are seeking the best CEL I producing cultivar of celery or a plant source better than celery to produce the enzyme. The expression of CEL I RNA was compared in some plants belong to the APIACEAE family using SEMI-QUANTITATIVE RT-PCR. The expression levels of the cel I gene were investigated in 12 plants including 7 cultivars of celery and five of its relative species from the APIACEAE famuly. The differentiall expression of the cel I gene was observed in both different celery cultivars and the plant species. Although 5 celery cultivars showed the same expression level, 2 cultivars, GSB and Victoria, showed a some lower expression. Differentially expression of cel I gene was more obvious among different APIACEAE family plants where Anethum graveolens and Foeniculum vulgare, showed considerably higher levels of cel I gene expression than celery.

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APA: Copy

ZOLALA, J., FARSI, MOHAMMAD, BAHRAMI, AHMAD REZA, MOGHADAM MATIN, M., & GHABOULI, M.. (2008). THE COMPARISON OF CEL I ENDONUCLEASE GENE EXPRESSION IN SOME APIACEAE PLANTS USING SEMI-QUANTITATIVE RT-PCR. JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), 22(1), 3-11. SID. https://sid.ir/paper/142236/en

Vancouver: Copy

ZOLALA J., FARSI MOHAMMAD, BAHRAMI AHMAD REZA, MOGHADAM MATIN M., GHABOULI M.. THE COMPARISON OF CEL I ENDONUCLEASE GENE EXPRESSION IN SOME APIACEAE PLANTS USING SEMI-QUANTITATIVE RT-PCR. JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY)[Internet]. 2008;22(1):3-11. Available from: https://sid.ir/paper/142236/en

IEEE: Copy

J. ZOLALA, MOHAMMAD FARSI, AHMAD REZA BAHRAMI, M. MOGHADAM MATIN, and M. GHABOULI, “THE COMPARISON OF CEL I ENDONUCLEASE GENE EXPRESSION IN SOME APIACEAE PLANTS USING SEMI-QUANTITATIVE RT-PCR,” JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), vol. 22, no. 1, pp. 3–11, 2008, [Online]. Available: https://sid.ir/paper/142236/en

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