STUDYING THE EFFICIENCY OF FLORAL DIP METHOD FOR GENETIC TRANSFORMATION OF APIACEAEPLANTS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHABOULI M.; BAHRAMI A.R.; SHAHRIARI F.A.; ZOLALA J.; MOHAMMADI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Horticultural Science Year:2013 | Volume:27 | Issue:2 Page(s): 139-147

Download Full-Text

Persian Verion

View:

1,408

Download:

Cites:

Information Journal Paper

Title

STUDYING THE EFFICIENCY OF FLORAL DIP METHOD FOR GENETIC TRANSFORMATION OF APIACEAEPLANTS

Author(s)

Keywords

FLORAL DIPQ2

PLANT TRANSFORMATIONQ2

APIACEAEQ3

ARABIDOPSISQ2

AGROBACTERIUMQ2

Abstract

Plant tissue culture techniques are used as basic requirement of common PLANT TRANSFORMATION systems. In most cases of PLANT TRANSFORMATION, a reproducible regeneration protocol is the limiting step due to long time lasting, specialized facilities and well experienced persons. Furthermore, tissue culture procedures induce somaclonal variation among regenerated transgenic plants. Therefore, recently current studies in plant molecular biology prefer PLANT TRANSFORMATION procedures avoiding tissue culture phase. Various in plant a transformation procedures have been explored, among which the FLORAL DIP method is the most reliablein vivo transformation method. In this research, with the aim of evaluating the ability of FLORAL DIP method for genetic transformation of someApiaceae plants, we studied Dill (Anethum graveolens), Fennel (Foeniculum vulgare), Coriander (Coriandrum sativum), Carrot (Daucus carrota), Parsley (Petrocelium sativum) and Celery (Apium graveolens).Arabidopsis thalianawas used as a model plant of experimental procedure. Flowers, in different stages of inflorescence development, were immersed in different suspension ofAgrobacterium tumefaciens carrying the plant binary vector pBI121. This vector carries plant reporter geneuidA (gus) and the plant selectable marker genenpt II. Although, producing transgenic ARABIDOPSIS plants with a high transformation rate of 4% verified the accuracy of experimental procedure, FLORAL DIP method was not successful for transformation ofApiaceae plants. Only one transformed celery plantlet, carryingnpt II gene with no expression of GUS, was obtained bby screening more than 10000 seeds produced by treated plants from all the species. Transgenic ARABIDOPSIS plants expressinggus reporter gene were confirmed through PCR and histochemical assays.

Cites

No record.

References

No record.

Cite

APA: Copy

GHABOULI, M., BAHRAMI, A.R., SHAHRIARI, F.A., ZOLALA, J., & MOHAMMADI, A.. (2013). STUDYING THE EFFICIENCY OF FLORAL DIP METHOD FOR GENETIC TRANSFORMATION OF APIACEAEPLANTS. JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), 27(2), 139-147. SID. https://sid.ir/paper/142256/en

Vancouver: Copy

GHABOULI M., BAHRAMI A.R., SHAHRIARI F.A., ZOLALA J., MOHAMMADI A.. STUDYING THE EFFICIENCY OF FLORAL DIP METHOD FOR GENETIC TRANSFORMATION OF APIACEAEPLANTS. JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY)[Internet]. 2013;27(2):139-147. Available from: https://sid.ir/paper/142256/en

IEEE: Copy

M. GHABOULI, A.R. BAHRAMI, F.A. SHAHRIARI, J. ZOLALA, and A. MOHAMMADI, “STUDYING THE EFFICIENCY OF FLORAL DIP METHOD FOR GENETIC TRANSFORMATION OF APIACEAEPLANTS,” JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), vol. 27, no. 2, pp. 139–147, 2013, [Online]. Available: https://sid.ir/paper/142256/en

Related Journal Papers