TYPE AND CONCENTRATION ROLE OF SUGAR IN SOMATIC EMBRYOGENESIS OF CARNATION (DIANTHUS CARYOPHYLLUS L.) CULTIVAR OF NELSON

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KARAMI O.; DELJOU A.; BAHMANI R.

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Journal: Journal of Horticultural Science Year:2008 | Volume:22 | Issue:1 Page(s): 13-21

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Title

TYPE AND CONCENTRATION ROLE OF SUGAR IN SOMATIC EMBRYOGENESIS OF CARNATION (DIANTHUS CARYOPHYLLUS L.) CULTIVAR OF NELSON

Author(s)

KARAMI O. | DELJOU A. | BAHMANI R. | Issue Writer Certificate

Keywords

CARNATIONQ3

SOMATIC EMBRYOGENESISQ3

EMBRYOGENIC CALLUSQ3

SUGARQ3

Abstract

In this study, the effect of different concentrations of sucrose, maltose, glucose and fructose on induction of EMBRYOGENIC CALLUS and somatic embryos of one CARNATION cultivar (Nelson) were investigated. Embryogenic calli were produced on MS culture medium containing four concentrations of sucrose (30, 6o, 90 and 120 g/l-1) all supplemented with 2 mg/l 2,4-D and 0.2 mg/l BA. Maximum frequency of EMBRYOGENIC CALLUS was obtained from the media containing 9% sucrose. Somatic embryos were formed when EMBRYOGENIC CALLUS were transferred to MS medium without growth regulators containing 15, 30, 60, 90 and 120 g/l-1 sucrose, maltose, glucose and fructose. Normal embryos were not developed in the media containing 15 and 30 g/l-1 sucrose, maltose, glucose and fructose. Somatic embryos were transferred to half-strength hormone-free MS culture medium containing 30 gl-1 sucrose, were germinated into plantlets about %90. Plantlets also continued their growth under greenhouse conditions.

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APA: Copy

KARAMI, O., DELJOU, A., & BAHMANI, R.. (2008). TYPE AND CONCENTRATION ROLE OF SUGAR IN SOMATIC EMBRYOGENESIS OF CARNATION (DIANTHUS CARYOPHYLLUS L.) CULTIVAR OF NELSON. JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), 22(1), 13-21. SID. https://sid.ir/paper/142240/en

Vancouver: Copy

KARAMI O., DELJOU A., BAHMANI R.. TYPE AND CONCENTRATION ROLE OF SUGAR IN SOMATIC EMBRYOGENESIS OF CARNATION (DIANTHUS CARYOPHYLLUS L.) CULTIVAR OF NELSON. JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY)[Internet]. 2008;22(1):13-21. Available from: https://sid.ir/paper/142240/en

IEEE: Copy

O. KARAMI, A. DELJOU, and R. BAHMANI, “TYPE AND CONCENTRATION ROLE OF SUGAR IN SOMATIC EMBRYOGENESIS OF CARNATION (DIANTHUS CARYOPHYLLUS L.) CULTIVAR OF NELSON,” JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), vol. 22, no. 1, pp. 13–21, 2008, [Online]. Available: https://sid.ir/paper/142240/en

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