THE STUDY OF INFECTION AND DISTRIBUTION OF BEET MOSAIC VIRUS IN KHORASAN RAZAVI PROVINCE’S FIELDS

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GERAYELI N.; JAFARPOUR B.; FALAHATI RASTEGAR M.; ZABIHNIA MOGHADDAM A.

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Journal: Iranian Plant Protection Research Year:2010 | Volume:24 | Issue:2 Page(s): 122-127

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Title

THE STUDY OF INFECTION AND DISTRIBUTION OF BEET MOSAIC VIRUS IN KHORASAN RAZAVI PROVINCE’S FIELDS

Author(s)

GERAYELI N. | JAFARPOUR B. | FALAHATI RASTEGAR M. | ZABIHNIA MOGHADDAM A. | Issue Writer Certificate

Keywords

BEET MOSAIC VIRUSQ3

DAS-ELISAQ2

KHORASANQ2

RT-PCRQ2

Abstract

Razavi KHORASAN province is one of the most important sugar beet growing regions in Iran. BEET MOSAIC VIRUS (BtMV) is one of the important viruses of sugar beet and is widespread on sugar beet all over the world. This virus causes mosaic, mottling pattern and curling of leaves. A survey was conducted in summer of 2007 to detect and determining distribution of BtMV in Razavi KHORASAN province. To achieved these goals some samples were collected randomly and also some based on apparent symptoms from sugar beet fields located in Mashhad, Neyshabour, Ghoochan, Torbat-e-heydariyeh, Torbat-e-jam, Fariman and Kashmar. All samples were tested by DAS- ELISA to find infected samples. In order to carry out molecular tests, virus RNA was extracted from infected leaves using PEG 6000 percipitation method. To performed RT-PCR viral cDNA was synthesized using specific primers. PCR products was run on %1 Agarose gel and the band of 658 bp related to the virus was observed. Presence of this amplified region in the samples confirmed that BtMV infection exists in collected samples. Our survey showed that infection exists in different percentage all tested fields. Fariman (with %33.4) and Neyshabour (with %4.8) have maximum and minimum infection rate respectively. Average infection percentage in Razavi KHORASAN province (with 530 collected samples) was %14.9.

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APA: Copy

GERAYELI, N., JAFARPOUR, B., FALAHATI RASTEGAR, M., & ZABIHNIA MOGHADDAM, A.. (2010). THE STUDY OF INFECTION AND DISTRIBUTION OF BEET MOSAIC VIRUS IN KHORASAN RAZAVI PROVINCE’S FIELDS. IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION), 24(2), 122-127. SID. https://sid.ir/paper/142588/en

Vancouver: Copy

GERAYELI N., JAFARPOUR B., FALAHATI RASTEGAR M., ZABIHNIA MOGHADDAM A.. THE STUDY OF INFECTION AND DISTRIBUTION OF BEET MOSAIC VIRUS IN KHORASAN RAZAVI PROVINCE’S FIELDS. IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION)[Internet]. 2010;24(2):122-127. Available from: https://sid.ir/paper/142588/en

IEEE: Copy

N. GERAYELI, B. JAFARPOUR, M. FALAHATI RASTEGAR, and A. ZABIHNIA MOGHADDAM, “THE STUDY OF INFECTION AND DISTRIBUTION OF BEET MOSAIC VIRUS IN KHORASAN RAZAVI PROVINCE’S FIELDS,” IRANIAN PLANT PROTECTION RESEARCH (JOURNAL OF PLANT PROTECTION), vol. 24, no. 2, pp. 122–127, 2010, [Online]. Available: https://sid.ir/paper/142588/en

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