Tracing and introducing SSR markers in Cannabis sativa by exploring in transcriptome

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Soreni Abouzar; SALAMI SEYED ALIREZA; FATTAHI MOGHADDAM MOHAMMAD REZA

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Journal: Iranian Journal of Horticultural Science Year:2018 | Volume:49 | Issue:2 Page(s): 313-321

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Information Journal Paper

Title

Tracing and introducing SSR markers in Cannabis sativa by exploring in transcriptome

Author(s)

Soreni Abouzar | SALAMI SEYED ALIREZA | FATTAHI MOGHADDAM MOHAMMAD REZA | Issue Writer Certificate

Keywords

Dinucleotide motifsQ1

fiber and drug typesQ1

genetic researchQ1

microsatellitesQ1

Abstract

Cannabis sativa L. is an important economic plant for the production of medical, food, fiber and oils. Nevertheless, lack of sufficient simple sequence repeat (SSR) markers has limited the development of cannabis genetic research. In this study, transcriptome sequences were used for identification and introduction of SSR markers in order to assess genetic diversity and separation of fiber and drug types. Based on the cannabis RNA-Seq data, 3383 SSR were identified from 1402 reads in fiber type whiles this number was 10381 for 4743 reads in drug type. Among these markers, trinucleotide repeat motifs were the most abundant, followed by mononucleotide and dinucleotide. Primers were successfully designed for amplification of 234 SSR markers from 152 individual sequences in fiber and 1543 SSR markers from 1372 individual sequences in drug using Primer3 with default parameters. This study outlines the first large-scale development of SSR markers for cannabis. The effectiveness of these molecular markers should be tested using different Iranian Marijuana populations and could be particularly useful for cannabis populations breeding.

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APA: Copy

Soreni, Abouzar, SALAMI, SEYED ALIREZA, & FATTAHI MOGHADDAM, MOHAMMAD REZA. (2018). Tracing and introducing SSR markers in Cannabis sativa by exploring in transcriptome. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 49(2 ), 313-321. SID. https://sid.ir/paper/148070/en

Vancouver: Copy

Soreni Abouzar, SALAMI SEYED ALIREZA, FATTAHI MOGHADDAM MOHAMMAD REZA. Tracing and introducing SSR markers in Cannabis sativa by exploring in transcriptome. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2018;49(2 ):313-321. Available from: https://sid.ir/paper/148070/en

IEEE: Copy

Abouzar Soreni, SEYED ALIREZA SALAMI, and MOHAMMAD REZA FATTAHI MOGHADDAM, “Tracing and introducing SSR markers in Cannabis sativa by exploring in transcriptome,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 49, no. 2 , pp. 313–321, 2018, [Online]. Available: https://sid.ir/paper/148070/en

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