SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN GRAPEVINE (VITIS VINIFERA L.) CVS YAGHOUTI, BIDANE SEFID, SHAHROODI AND FLAME SEEDLESS USING OVARY EXPLANT

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JAMALMAHMOOD AMIR; EBADI ALI; MIRMASOUMI MASOUD; OMIDI MANSOUR

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Journal: Iranian Journal of Horticultural Science Year:2015 | Volume:45 | Issue:4 Page(s): 345-352

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Title

SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN GRAPEVINE (VITIS VINIFERA L.) CVS YAGHOUTI, BIDANE SEFID, SHAHROODI AND FLAME SEEDLESS USING OVARY EXPLANT

Author(s)

JAMALMAHMOOD AMIR | EBADI ALI | MIRMASOUMI MASOUD | OMIDI MANSOUR | Issue Writer Certificate

Keywords

GRAPEVINEQ2

OVARY EXPLANTQ2

REGENERATIONQ1

SOMATIC EMBRYOGENESISQ2

Abstract

To produce embryonic callus in four GRAPEVINE cultivars i.e. Yaghouti, Bidane Sefid, Shahroodi and Flame Seedless, OVARY EXPLANTs were collected at flower developmental stages III and V and cultured in MS medium supplemented with various concentrations of 2,4-D and BAP. For differentiation of somatic embryos, MS medium was supplemented with various concentrations of IAA and BAP. Results showed that collecting ovaries explants at stage V produced the highest percentage of somatic embryos in all studied cultivars. For embryogenic callus production, MS medium supplemented with 4.5μm 2,4-D and 1.1μm BAP was best treatment. However, for embryo differentiation, MS medium supplemented with 2mg/l IAA and 1mg/l BAP was better than the other treatments. The highest percentage of callus which produced somatic embryos obtained in ‛Yaghouti’ and ‛Bidaneh Sefid’ at second sampling time (V), whereas the lowest percentage of callus producing somatic embryos was achieved in ‛Shahroodi’ and ‛Flame Seedless’ at first sampling time (III). For germination and production of plantlets, embryos at the torpedo stage, were transferred to MS medium contained 1mg/l BAP. In this medium most of the embryos were germinated.

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APA: Copy

JAMALMAHMOOD, AMIR, EBADI, ALI, MIRMASOUMI, MASOUD, & OMIDI, MANSOUR. (2015). SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN GRAPEVINE (VITIS VINIFERA L.) CVS YAGHOUTI, BIDANE SEFID, SHAHROODI AND FLAME SEEDLESS USING OVARY EXPLANT. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 45(4), 345-352. SID. https://sid.ir/paper/148081/en

Vancouver: Copy

JAMALMAHMOOD AMIR, EBADI ALI, MIRMASOUMI MASOUD, OMIDI MANSOUR. SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN GRAPEVINE (VITIS VINIFERA L.) CVS YAGHOUTI, BIDANE SEFID, SHAHROODI AND FLAME SEEDLESS USING OVARY EXPLANT. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2015;45(4):345-352. Available from: https://sid.ir/paper/148081/en

IEEE: Copy

AMIR JAMALMAHMOOD, ALI EBADI, MASOUD MIRMASOUMI, and MANSOUR OMIDI, “SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN GRAPEVINE (VITIS VINIFERA L.) CVS YAGHOUTI, BIDANE SEFID, SHAHROODI AND FLAME SEEDLESS USING OVARY EXPLANT,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 45, no. 4, pp. 345–352, 2015, [Online]. Available: https://sid.ir/paper/148081/en

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