Effect of culture medium and growth regulator in meristem culture of sweet Cherry

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Naddaf Mohammad Esmaeil; RABIEI GHOLAMREZA; GANJI MOGHADAM EBRAHIM; Mohammadkhani Abdorahman

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Journal: Iranian Journal of Horticultural Science Year:2019 | Volume:50 | Issue:3 Page(s): 587-594

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Title

Effect of culture medium and growth regulator in meristem culture of sweet Cherry

Author(s)

Naddaf Mohammad Esmaeil | RABIEI GHOLAMREZA | GANJI MOGHADAM EBRAHIM | Mohammadkhani Abdorahman | Issue Writer Certificate

Keywords

Multiplication rateQ1

propagationQ1

shoot tipsQ1

surviving rateQ1

virus-freeQ1

Abstract

Regeneration through meristem culture is an efficient way to obtain virus-free plants. This study was conducted to effect of the medium and growth regulator on meristem cultivation in sweet cherry cultivars with factorial experiment based on completely randomized design with seven cultivars (Zard, Siah-E-Mashhad, Dovomres, Bing, Pishres, Takdaneh and Hajyousefi) and type of treatment in four levels of medium and growth regulator (M1H1: half MS without growth regulator, M2H1: complete MS without growth regulator, M1H2: half MS with growth regulator, M2H2: complete MS with growth regulator). Meristem explants (less than 1 mm) were isolated from shoot tips and were cultured in MS medium under controlled conditions; after third subculture (sixth week), meristem survival and activation After sixth subculture (third month), the Multiplication rate (production shoot to active sample) and length of the shoot were measured. The results showed that there was no significant difference in survival index as well as Multiplication rate and shoot length between cultivars, but this difference was significant between treatments. The highest survival percentage was obtained for Hajyosefi (52. 4%), Multiplication rate for Dovomres (2. 2) and shoot length for Bing (7. 9 cm) cultivar in MS medium with 1mg. l-1 BA growth regulator.

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APA: Copy

Naddaf, Mohammad Esmaeil, RABIEI, GHOLAMREZA, GANJI MOGHADAM, EBRAHIM, & Mohammadkhani, Abdorahman. (2019). Effect of culture medium and growth regulator in meristem culture of sweet Cherry. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 50(3 ), 587-594. SID. https://sid.ir/paper/148185/en

Vancouver: Copy

Naddaf Mohammad Esmaeil, RABIEI GHOLAMREZA, GANJI MOGHADAM EBRAHIM, Mohammadkhani Abdorahman. Effect of culture medium and growth regulator in meristem culture of sweet Cherry. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2019;50(3 ):587-594. Available from: https://sid.ir/paper/148185/en

IEEE: Copy

Mohammad Esmaeil Naddaf, GHOLAMREZA RABIEI, EBRAHIM GANJI MOGHADAM, and Abdorahman Mohammadkhani, “Effect of culture medium and growth regulator in meristem culture of sweet Cherry,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 50, no. 3 , pp. 587–594, 2019, [Online]. Available: https://sid.ir/paper/148185/en

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