In vitro propagation of Persian lime (Citrus latifolia)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOJTAHEDI NARGES

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Horticultural Science Year:2017 | Volume:48 | Issue:2 Page(s): 381-389

Download Full-Text

Persian Verion

View:

636

Download:

Cites:

Information Journal Paper

Title

In vitro propagation of Persian lime (Citrus latifolia)

Author(s)

MOJTAHEDI NARGES | Issue Writer Certificate

Keywords

AcclimatizationQ2

elongationQ1

limeQ2

micropropagationQ2

proliferationQ1

rootingQ2

Abstract

Application of tolerant and resistant rootstocks and varieties is the best way to overcome Witches’ Broom Disease of lime (WBDL). The aim of this research was to develop a practical protocol for micropropagtaion of Persian lime (Citrus latifolia) as one of the highly tolerant varieties. For culture establishment, apical and axillary buds of young stems of three-year-old trees were cultured in free hormone MS medium after surface sterilization. The explants were transferred to DKW and MS culture media containing different concentrations of BAP for proliferation after 20-30 days. Another experiment was carried out using Kinetin and BAP for determination of shoot elongation medium. Two separated experiments using three concentrations of IAA and NAA and three concentrations of IBA and NAA in DKW medium and 2% sucrose were used to obtain the best medium for rooting. To acquire the suitable soil mixture for Acclimatization, an experiment using six different soil mixtures was done. The results showed that DKW basal culture medium supplemented with Gamborge’ s B5 vitamins, 1 mg. L-1 BAP and 3% sucrose was the best medium for proliferation. DKW basal culture medium supplemented with Gamborge’ s B5 vitamins and 0. 25 mg. L-1 BAP was the best medium for elongation. The best rooting medium was DKW basal culture medium, Gamborge’ s B5 vitamins, 2% sucrose and 2 mg. L-1 NAA. The optimum soil mixture with 88% of survival for acclimating in vitro plantlets was sand: perlite: peat: clay (1: 1: 3: 3). The plantlets could transfer to the orchard after 60 days.

Cites

No record.

References

No record.

Cite

APA: Copy

MOJTAHEDI, NARGES. (2017). In vitro propagation of Persian lime (Citrus latifolia). IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 48(2 ), 381-389. SID. https://sid.ir/paper/148307/en

Vancouver: Copy

MOJTAHEDI NARGES. In vitro propagation of Persian lime (Citrus latifolia). IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2017;48(2 ):381-389. Available from: https://sid.ir/paper/148307/en

IEEE: Copy

NARGES MOJTAHEDI, “In vitro propagation of Persian lime (Citrus latifolia),” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 48, no. 2 , pp. 381–389, 2017, [Online]. Available: https://sid.ir/paper/148307/en

Related Journal Papers