CRYOPRESERVATION OF POPULUS EUPHRATICA OLIV. SEEDS AND EVALUATION OF THE CRYOPRESERVED SEEDS UNDER LABORATORY AND GREENHOUSE CONDITIONS

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JEBELI MARYAM; NADERI SHAHAB MOHABAT ALI; FEIZI HAMID REZA

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Journal: Iranian Journal of Horticultural Science Year:2015 | Volume:46 | Issue:2 Page(s): 313-322

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Information Journal Paper

Title

CRYOPRESERVATION OF POPULUS EUPHRATICA OLIV. SEEDS AND EVALUATION OF THE CRYOPRESERVED SEEDS UNDER LABORATORY AND GREENHOUSE CONDITIONS

Author(s)

JEBELI MARYAM | NADERI SHAHAB MOHABAT ALI | FEIZI HAMID REZA | Issue Writer Certificate

Keywords

SEEDQ2

POPULUS EUPHRATICAQ2

CRYOPRESERVATIONQ2

DESICCATIONQ2

LIQUID NITROGENQ2

Abstract

Populus euphratica Oliv. is a highly heat, saline and cold tolerant native species, with wide range of ecological habitats and conditions. SEED longevity of P. euphratica is too short to be stored in traditional gene banks. For evaluating preservation of P. euphratica SEEDs in normal conditions (+22oC) as well as in cryogenic conditions (-196oC), three pre-cryopreservation treatments including PVS2, DESICCATION and 30% Glycerol were applied before transferring the SEEDs into LIQUID NITROGEN (-196oC) for 1 week, 1 month and 1 year. To evaluate the effects of the pre-treatments on SEED germination and establishment, a part of the SEEDs were treated with PVS2, DESICCATION and 30%Glycerol and incubated at +22oC, instead of LIQUID NITROGEN (-196oC). The 30%Glycerol and PVS2 pre-treatments dramatically reduced the SEED germination from 90% in control SEEDs to 35.00 and 41.67%. Cryogenic (-196oC) condition reduced SEED germination from 90% in control SEEDs to 26.67% and 28.33. However, Desication showed excellent protection of SEEDs in cryogenic conditions in SEED germination and establishment in lab and greenhouse. Control SEED in normal conditions stayed for few months. Germination of cryopreserved SEEDs for a period of one year did not show significant reduction, compared to those kept for one week or one month at -196oC.

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APA: Copy

JEBELI, MARYAM, NADERI SHAHAB, MOHABAT ALI, FEIZI, HAMID REZA,. (2015). CRYOPRESERVATION OF POPULUS EUPHRATICA OLIV. SEEDS AND EVALUATION OF THE CRYOPRESERVED SEEDS UNDER LABORATORY AND GREENHOUSE CONDITIONS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 46(2), 313-322. SID. https://sid.ir/paper/148343/en

Vancouver: Copy

JEBELI MARYAM, NADERI SHAHAB MOHABAT ALI, FEIZI HAMID REZA. CRYOPRESERVATION OF POPULUS EUPHRATICA OLIV. SEEDS AND EVALUATION OF THE CRYOPRESERVED SEEDS UNDER LABORATORY AND GREENHOUSE CONDITIONS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2015;46(2):313-322. Available from: https://sid.ir/paper/148343/en

IEEE: Copy

MARYAM JEBELI, MOHABAT ALI NADERI SHAHAB, HAMID REZA FEIZI, an, “CRYOPRESERVATION OF POPULUS EUPHRATICA OLIV. SEEDS AND EVALUATION OF THE CRYOPRESERVED SEEDS UNDER LABORATORY AND GREENHOUSE CONDITIONS,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 46, no. 2, pp. 313–322, 2015, [Online]. Available: https://sid.ir/paper/148343/en

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