AN EVALUATION OF GENETIC DIVERSITY IN SOME POPULATIONS OF BLACK CUMMIN (BUNIUM PERSICUM) USING RAPD MARKERS

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PEZHMAN MEHR M.; HASANI M.E.; FAKHR TABATABAEI S.M.

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Journal: Iranian Journal of Horticultural Science Year:2008 | Volume:39 | Issue:1 Page(s): 57-65

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Title

AN EVALUATION OF GENETIC DIVERSITY IN SOME POPULATIONS OF BLACK CUMMIN (BUNIUM PERSICUM) USING RAPD MARKERS

Author(s)

PEZHMAN MEHR M. | HASANI M.E. | FAKHR TABATABAEI S.M. | Issue Writer Certificate

Keywords

GENETIC DIVERSITYQ2

BUNIUM PERSICUMQ3

RAPD MARKERSQ3

Abstract

Black cummin, BUNIUM PERSICUM (Boiss) is a native medicinal plant of Iran with a considerable distribution throughout the country. Regarding the necessity of a thorough study towards domestication of this valuable medicinal plant, in this research, GENETIC DIVERSITY of twenty four populations of black cummin was studied using RAPD MARKERS. Fifteen RAPD primers of the highest level of reproducibility of template DNA and polymorphism among the banding pattern of the samples were selected for the study of populations. Two hundred and twenty eight bands were produced from among which one hundred and ninety six (86%) were polymorphic. Average polymorphism obtained for each RAPD primers was 13.97. Jaccard's similarity coefficient was used for an assessment of genetic similarities among populations. Estimates of genetic similarity based on the polymorphic bands ranged from 0.19 to 0.75 with an average of 0.53. Cluster analysis was performed based on similarity matrix and an application of UPGMA method. In the resultant dendrogram, maximum similarity was detected between Sirch and Jiroft-Darzin while minimum similarity observed between Barn, and Sistan & Baluchestan populations, the results showed a high level of GENETIC DIVERSITY among investigated populations, while also indicating that RAPD technique is an efficient tool for assessing GENETIC DIVERSITY in black cummin.

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APA: Copy

PEZHMAN MEHR, M., HASANI, M.E., & FAKHR TABATABAEI, S.M.. (2008). AN EVALUATION OF GENETIC DIVERSITY IN SOME POPULATIONS OF BLACK CUMMIN (BUNIUM PERSICUM) USING RAPD MARKERS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 39(1), 57-65. SID. https://sid.ir/paper/148374/en

Vancouver: Copy

PEZHMAN MEHR M., HASANI M.E., FAKHR TABATABAEI S.M.. AN EVALUATION OF GENETIC DIVERSITY IN SOME POPULATIONS OF BLACK CUMMIN (BUNIUM PERSICUM) USING RAPD MARKERS. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2008;39(1):57-65. Available from: https://sid.ir/paper/148374/en

IEEE: Copy

M. PEZHMAN MEHR, M.E. HASANI, and S.M. FAKHR TABATABAEI, “AN EVALUATION OF GENETIC DIVERSITY IN SOME POPULATIONS OF BLACK CUMMIN (BUNIUM PERSICUM) USING RAPD MARKERS,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 39, no. 1, pp. 57–65, 2008, [Online]. Available: https://sid.ir/paper/148374/en

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