AN EVALUATION OF GENETIC DIVERSITY AMONG SOME POMEGRANATE GENOTYPES USING RAPD MARKERS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ZAMANI Z.; SARKHOUSH A.; FATAHI MOGHADAM M.R.; EBADI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE) Year:2006 | Volume:37 | Issue:5 Page(s): 865-874

Download Full-Text

Persian Verion

View:

989

Download:

Cites:

Information Journal Paper

Title

AN EVALUATION OF GENETIC DIVERSITY AMONG SOME POMEGRANATE GENOTYPES USING RAPD MARKERS

Author(s)

ZAMANI Z. | SARKHOUSH A. | FATAHI MOGHADAM M.R. | EBADI A. | Issue Writer Certificate

Keywords

POMEGRANATEQ3

GENETIC DIVERSITYQ2

MOLECULAR MARKERSQ2

RAPDQ3

PCRQ3

Abstract

Iran is in possession of the richest gene pool of POMEGRANATE, with an amazing diversity and number of genotypes, in the world. In this study RAPD markers were mployed to determine the level of diversity, and phylogenetic relationships among 24 Iranian POMEGRANATE genotypes. Genomic DNA extracted from leaves, and its quantity as well as quality were determined by use of spectrophotometry and agarose gel electrophoresis to be used for PCR after being adjusted for the concentrations. In total 113 primers were used for PCR reactions on the template DNAs among which 27 showed reasonable amplification and polymorphism. Bands with a acceptable resolution and high repeatability were selected for calculations. These 27 primers produced 257 bands, among them, 158 were polymorphic. Cluster analysis of the genotypes was performed based on the presence (1) or absence (0) of the bands, using Jaccard's similarity coefficient and UPGMA method. The highest and lowest observed genetic similarity between genotypes was 0.88 and 0.30 respectively. At a distance of 0.64 similarities on the dendrogram, the genotypes were divided into 8 sub.clusters. A Cophenetic coefficient of r=0.92 between similarity matrices and the dendrogram is an indication of the dendrogram properly fitting the similarity data. The examined genotypes were to relatively high diversity, implying the richness of POMEGRANATE genetic resources in Iran. Furthermore, this study reflected the possibility of similar names \ having been given to different genotypesor the mistakes and mixes in either collection establishments or during translocation of genotypes. Besides, RAPD markers were shown to be a useful technique for studying the GENETIC DIVERSITY in POMEGRANATE.

Cites

STUDY OF GENETIC DIVERSITY OF EXOTIC AND ENDEMIC DAFFODILS (NARCISSUS SPP.) USING RAPD MARKERS

References

No record.

Cite

APA: Copy

ZAMANI, Z., SARKHOUSH, A., FATAHI MOGHADAM, M.R., & EBADI, A.. (2006). AN EVALUATION OF GENETIC DIVERSITY AMONG SOME POMEGRANATE GENOTYPES USING RAPD MARKERS. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), 37(5), 865-874. SID. https://sid.ir/paper/8561/en

Vancouver: Copy

ZAMANI Z., SARKHOUSH A., FATAHI MOGHADAM M.R., EBADI A.. AN EVALUATION OF GENETIC DIVERSITY AMONG SOME POMEGRANATE GENOTYPES USING RAPD MARKERS. IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE)[Internet]. 2006;37(5):865-874. Available from: https://sid.ir/paper/8561/en

IEEE: Copy

Z. ZAMANI, A. SARKHOUSH, M.R. FATAHI MOGHADAM, and A. EBADI, “AN EVALUATION OF GENETIC DIVERSITY AMONG SOME POMEGRANATE GENOTYPES USING RAPD MARKERS,” IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), vol. 37, no. 5, pp. 865–874, 2006, [Online]. Available: https://sid.ir/paper/8561/en

Related Journal Papers