PURIFICATION OF CONJUGATED LINOLEIC ACID ISOMERS BY SELECTIVE ESTERIFICATION WITH CANDIDA RUGOSA LIPASE

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JAFARI M.; KADIVAR M.; GOLI S.A.H.

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Journal Paper

Paper Information

Journal: JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ) Year:2015 | Volume:24 | Issue:4 Page(s): 497-506

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Title

PURIFICATION OF CONJUGATED LINOLEIC ACID ISOMERS BY SELECTIVE ESTERIFICATION WITH CANDIDA RUGOSA LIPASE

Author(s)

JAFARI M. | KADIVAR M. | GOLI S.A.H. | Issue Writer Certificate

Keywords

CANDIDA RUGOSAQ2

CONJUGATED LINOLEIC ACIDQ2

L-MENTHOLQ2

ESTERIFICATIONQ2

Abstract

Ability of CANDIDA RUGOSA for separation of two main CONJUGATED LINOLEIC ACID (CLA) isomers including 9-cis, 11-trans and 10-trans, 12-cis was investigated in the mixture of free fatty acids of these isomers and L-MENTHOL. In order to effectively separate two mentioned isomers, optimization of reaction conditions was conducted using response surface methodology (RSM). After data analysis by D-optimal design, the best reaction conditions for maximum ESTERIFICATION of 9-cis, 11-trans isomer with L-MENTHOL after just one step ESTERIFICATION were as follows: reaction time 23.1 h, temperature 32.6oC, enzyme amount 135.4U, molar ratio of CLA oil to L-MENTHOL at 1: 1.7 and pH at 7.7. At this optimum point, the lowest amount of c9, t11 -CLA isomer (6.7%) was observed in free fatty acids fraction. This effective process for purification of CLA isomers using L-MENTHOL could be applicable to produce food supplements.

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APA: Copy

JAFARI, M., KADIVAR, M., & GOLI, S.A.H.. (2015). PURIFICATION OF CONJUGATED LINOLEIC ACID ISOMERS BY SELECTIVE ESTERIFICATION WITH CANDIDA RUGOSA LIPASE. JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ), 24(4), 497-506. SID. https://sid.ir/paper/148565/en

Vancouver: Copy

JAFARI M., KADIVAR M., GOLI S.A.H.. PURIFICATION OF CONJUGATED LINOLEIC ACID ISOMERS BY SELECTIVE ESTERIFICATION WITH CANDIDA RUGOSA LIPASE. JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ)[Internet]. 2015;24(4):497-506. Available from: https://sid.ir/paper/148565/en

IEEE: Copy

M. JAFARI, M. KADIVAR, and S.A.H. GOLI, “PURIFICATION OF CONJUGATED LINOLEIC ACID ISOMERS BY SELECTIVE ESTERIFICATION WITH CANDIDA RUGOSA LIPASE,” JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ), vol. 24, no. 4, pp. 497–506, 2015, [Online]. Available: https://sid.ir/paper/148565/en

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