ISOLATION AND IDENTIFICATION OF LACTIC ACID BACTERIA IN MOTHER, S BREAST MILK USING 16S RIBOSOMAL DNA SEQUENCING METHOD

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TABATABAIE F.; ROOZBEH NASIRAII L.

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Paper Information

Journal: JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ) Year:2011 | Volume:21 | Issue:2 Page(s): 167-177

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Title

ISOLATION AND IDENTIFICATION OF LACTIC ACID BACTERIA IN MOTHER, S BREAST MILK USING 16S RIBOSOMAL DNA SEQUENCING METHOD

Author(s)

TABATABAIE F. | ROOZBEH NASIRAII L. | Issue Writer Certificate

Keywords

BREAST MILKQ2

POLYMERASE CHAIN REACTIONQ3

LACTIC ACID BACTERIAQ3

PROBIOTICQ2

Abstract

Human BREAST MILK is known to be the best food for rapidly growing infant scince breastfeeding protects the newborn against some diesease such as infection disease, asthma and allergy. This effect may be due to the useful and natural microflora of BREAST MILK which could be depending on diets of mothers. For the first time, this study accomplished to evaluate microflora of mother’s BREAST MILK along with sepration and identification of local bacteria that could be as a candidat for bacteriotherapy and possessing PROBIOTIC properties in Iran. In this study, 309 different colonies were isolated from 20 BREAST MILK samples of mothers who were registered in Mshhad. The isolated were selected based on the results of catalase test, gram stain, and bacterial morphology. The results showed that 40 out of 309 selected bacteria were catalase negative and gram positive stain. The whole genomes of 16s ribosomal DNA of isolated colonies were amplified using POLYMERASE CHAIN REACTION for identification down to the strain level. According to the results, the BREAST MILK contains various species of enterococci, Streptococci, Staphylococci and Lactobacilli. The most abundant bacteria, enterococcus faecium was isolated from 50% of samples representing 47.5% of the total isolated bacteria. Generally 10 and 27.5 percentage of isolated bacteria were La. rhamnosus Lc 705 and Streptococcus salivarius, respectively, that were isolated from 20 and 35 percent of milk samples, respectively.

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APA: Copy

TABATABAIE, F., & ROOZBEH NASIRAII, L.. (2011). ISOLATION AND IDENTIFICATION OF LACTIC ACID BACTERIA IN MOTHER, S BREAST MILK USING 16S RIBOSOMAL DNA SEQUENCING METHOD. JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ), 21(2), 167-177. SID. https://sid.ir/paper/148672/en

Vancouver: Copy

TABATABAIE F., ROOZBEH NASIRAII L.. ISOLATION AND IDENTIFICATION OF LACTIC ACID BACTERIA IN MOTHER, S BREAST MILK USING 16S RIBOSOMAL DNA SEQUENCING METHOD. JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ)[Internet]. 2011;21(2):167-177. Available from: https://sid.ir/paper/148672/en

IEEE: Copy

F. TABATABAIE, and L. ROOZBEH NASIRAII, “ISOLATION AND IDENTIFICATION OF LACTIC ACID BACTERIA IN MOTHER, S BREAST MILK USING 16S RIBOSOMAL DNA SEQUENCING METHOD,” JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ), vol. 21, no. 2, pp. 167–177, 2011, [Online]. Available: https://sid.ir/paper/148672/en

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