USE OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS TO DETECT GENETIC VARIATION OF NATIVE FOWLS IN MAZANDARAN NATIVE FOWLS BREEDING STATION

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KHAN AHMADI A.R.; RAHIMI GH.; NEJATI JAVAREMI A.; ESMAEIL KHANIAN S.

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Journal: Journal of Water and Soil Science Year:2007 | Volume:11 | Issue:40 (B) Page(s): 331-337

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Title

USE OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS TO DETECT GENETIC VARIATION OF NATIVE FOWLS IN MAZANDARAN NATIVE FOWLS BREEDING STATION

Author(s)

KHAN AHMADI A.R. | RAHIMI GH. | NEJATI JAVAREMI A. | ESMAEIL KHANIAN S. | Issue Writer Certificate

Keywords

MAZANDARAN NATIVE FOWLSQ3

RAPDQ3

GENETIC VARIATIONQ3

Abstract

In order to detect GENETIC VARIATION of native fowls in MAZANDARAN NATIVE FOWLS breeding station, blood samples were collected from 100 male and female of birds(1:11). The DNA of the blood samples was extracted according to an optimized salting out protocol. The extracted DNA was amplified through polymerase chain reaction (PCR). Of the twenty random primers (10 mer) were used in this study, fourteen yielded satisfactory PCR. The total 63 polymorphic and 77 monomorphic bands were detected for the 14 primers. The number of bands displayed for each primer ranged from 4 to 16 with 200-2100 base pairs. The highest and lowest percentages of polymorphism band were observed for primer 9 (72%) and primer 14 (16%) respectively. The band sharing frequency was calculated for each primer, which ranged from 79 to 96. The genetic similarity within population and GENETIC VARIATION were estimated as 89 and 11 percentage respectively. In conclusion, the existence of high level of polymorphism after ten generation of selection may indicate the accuracy of genetic evaluation program, suitable selection strategies and also large enough effective population size in this breeding flock.

Cites

بررسی تنوع و رابطه ژنتیکی شش توده مرغ بومی ایران با استفاده از نشانگرهای RAPD

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APA: Copy

KHAN AHMADI, A.R., RAHIMI, GH., NEJATI JAVAREMI, A., & ESMAEIL KHANIAN, S.. (2007). USE OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS TO DETECT GENETIC VARIATION OF NATIVE FOWLS IN MAZANDARAN NATIVE FOWLS BREEDING STATION. WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), 11(40 (B)), 331-337. SID. https://sid.ir/paper/15057/en

Vancouver: Copy

KHAN AHMADI A.R., RAHIMI GH., NEJATI JAVAREMI A., ESMAEIL KHANIAN S.. USE OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS TO DETECT GENETIC VARIATION OF NATIVE FOWLS IN MAZANDARAN NATIVE FOWLS BREEDING STATION. WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES)[Internet]. 2007;11(40 (B)):331-337. Available from: https://sid.ir/paper/15057/en

IEEE: Copy

A.R. KHAN AHMADI, GH. RAHIMI, A. NEJATI JAVAREMI, and S. ESMAEIL KHANIAN, “USE OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS TO DETECT GENETIC VARIATION OF NATIVE FOWLS IN MAZANDARAN NATIVE FOWLS BREEDING STATION,” WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), vol. 11, no. 40 (B), pp. 331–337, 2007, [Online]. Available: https://sid.ir/paper/15057/en

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