STUDY ON DNA POLYMORPHISM OF IRANIAN NATIVE CHICKENS POPULATION USING RAPD MARKERS

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DEHGHANZADEH H.; MIRHOSSEINI S.Z.A.D.; SHAD PARVAR A.A.A.

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Journal: Year:2004 | Volume:17 | Issue:1 (62 IN ANIMAL AND FISHERIES SCIENCES) Page(s): 2-9

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Title

STUDY ON DNA POLYMORPHISM OF IRANIAN NATIVE CHICKENS POPULATION USING RAPD MARKERS

Author(s)

DEHGHANZADEH H. | MIRHOSSEINI S.Z.A.D. | SHAD PARVAR A.A.A. | Issue Writer Certificate

Keywords

IRANIAN NATIVE CHICKENSQ4

GENETIC DIVERSITYQ2

POLYMORPHISMQ2

GENETIC DISTANCEQ2

RAPD MARKERQ4

Abstract

Genetic diversity of five IRANIAN NATIVE CHICKENS population from five province induding Mazandaran , Isfahan , Yazd, Fars and Azarbayjan were studied. From each population 30 birds randomly were selected and their blood samples were collected individually. The DNA of samples were extracted by modified salting-out method. The genomic DNA amplified by using 10 RAPD randomly primer through polymerase chain reaction (PCR). Length of amplified fragments by all primers was varied from 237 to 3240 base pairs. Based on polymorphic bands with 0.1819 as well as total bands with 0.1042, Fars and Mazandaran population had the highest GENETIC DISTANCE. On the other hand Fars and Isfahan population with 0.066 and 0.0391 had the lowest GENETIC DISTANCE Yazd and Mazandaran population with 0.208 and 0.156 had the highest and lowest inter population GENETIC DIVERSITY respectively. Based on phylogenic tree plotted by UPGMA method, chickens population were divided to two groups: Group I , including Isfahan, Fars, Yazd and Azarbayjan , and group II including Mazandaran only. Although there has not been found specific marker for each population in this study but we have found considerable differences in many loci. For instance locus of X03-5 the allele with 1853bp length, Isfahan, Fars , Yazd and Azarbayjan has frequency of I but in Mazandaran population has frequency of 0.225. With regard to low GENETIC DIVERSITY in population we can prevent the decreasing of GENETIC DIVERSITY by increasing of effective number of population, controlling mating system to obtaining the lowest inbreeding in each population and establishing separate base population with large number of chickens.

Cites

بررسی تنوع و رابطه ژنتیکی شش توده مرغ بومی ایران با استفاده از نشانگرهای RAPD

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APA: Copy

DEHGHANZADEH, H., MIRHOSSEINI, S.Z.A.D., & SHAD PARVAR, A.A.A.. (2004). STUDY ON DNA POLYMORPHISM OF IRANIAN NATIVE CHICKENS POPULATION USING RAPD MARKERS. PAJOUHESH-VA-SAZANDEGI, 17(1 (62 IN ANIMAL AND FISHERIES SCIENCES)), 2-9. SID. https://sid.ir/paper/19281/en

Vancouver: Copy

DEHGHANZADEH H., MIRHOSSEINI S.Z.A.D., SHAD PARVAR A.A.A.. STUDY ON DNA POLYMORPHISM OF IRANIAN NATIVE CHICKENS POPULATION USING RAPD MARKERS. PAJOUHESH-VA-SAZANDEGI[Internet]. 2004;17(1 (62 IN ANIMAL AND FISHERIES SCIENCES)):2-9. Available from: https://sid.ir/paper/19281/en

IEEE: Copy

H. DEHGHANZADEH, S.Z.A.D. MIRHOSSEINI, and A.A.A. SHAD PARVAR, “STUDY ON DNA POLYMORPHISM OF IRANIAN NATIVE CHICKENS POPULATION USING RAPD MARKERS,” PAJOUHESH-VA-SAZANDEGI, vol. 17, no. 1 (62 IN ANIMAL AND FISHERIES SCIENCES), pp. 2–9, 2004, [Online]. Available: https://sid.ir/paper/19281/en

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