REPORT OF CRYPTOSPORIDIUM ANDERSONI BASED ON HSP70 GENE AMPLIFICATION ISOLATED FROM SHEEP IN TEHRAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

DALIMI A.; TAHVILDAR F.; GHAFFARIFAR F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF VETERINARY MICROBIOLOGY Year:2017 | Volume:13 | Issue:2 (35) Page(s): 19-27

Download Full-Text

Persian Verion

View:

1,214

Download:

Cites:

Information Journal Paper

Title

REPORT OF CRYPTOSPORIDIUM ANDERSONI BASED ON HSP70 GENE AMPLIFICATION ISOLATED FROM SHEEP IN TEHRAN

Author(s)

DALIMI A. | TAHVILDAR F. | GHAFFARIFAR F. | Issue Writer Certificate

Keywords

CRYPTOSPORIDIUM ANDERSONIQ1

PARVUMQ1

HSP70 GENEQ1

NESTED-PCRQ2

SHEEPQ2

Abstract

Cryptosporidiosis is one of the most important parasitic infection in SHEEP. The aim of the present study, was to identify species of Cryptosporidium isolated from SHEEP in Tehran province based on HSP70 GENE by Nested PCR-RFLP assay. In the first step 1485 faecal samples were collected form SHEEP in Tehran province then the samples were examined for Cryptosporidium detection using modified acid fast staining. In the second step DNA of the positive samples were extracted, then gene ofHSP70 was amplified by NESTED-PCR in order to differentiate between species. The PCR product was digested by Hind II restriction enzyme. According to the result, 22 positive SHEEP samples were detected by modified acid fast method. The results were confirmed by molecular technique. The 800 bp fragment of HSP70 digested by restriction enzymes. Twenty samples showed similar band on 2.5% agarose gel whereas 2 samples demonstrated different pattern. Based on sequence results, the first and second groups were identified as CRYPTOSPORIDIUM ANDERSONIand C.parvum respectively. Generally, in spite of Cryptosporidium PARVUM introduced as the major agent of cryptosporidiosis in SHEEP but in our study CRYPTOSPORIDIUM ANDERSONI was dominant.

Cites

No record.

References

No record.

Cite

APA: Copy

DALIMI, A., TAHVILDAR, F., & GHAFFARIFAR, F.. (2017). REPORT OF CRYPTOSPORIDIUM ANDERSONI BASED ON HSP70 GENE AMPLIFICATION ISOLATED FROM SHEEP IN TEHRAN. JOURNAL OF VETERINARY MICROBIOLOGY, 13(2 (35) ), 19-27. SID. https://sid.ir/paper/152079/en

Vancouver: Copy

DALIMI A., TAHVILDAR F., GHAFFARIFAR F.. REPORT OF CRYPTOSPORIDIUM ANDERSONI BASED ON HSP70 GENE AMPLIFICATION ISOLATED FROM SHEEP IN TEHRAN. JOURNAL OF VETERINARY MICROBIOLOGY[Internet]. 2017;13(2 (35) ):19-27. Available from: https://sid.ir/paper/152079/en

IEEE: Copy

A. DALIMI, F. TAHVILDAR, and F. GHAFFARIFAR, “REPORT OF CRYPTOSPORIDIUM ANDERSONI BASED ON HSP70 GENE AMPLIFICATION ISOLATED FROM SHEEP IN TEHRAN,” JOURNAL OF VETERINARY MICROBIOLOGY, vol. 13, no. 2 (35) , pp. 19–27, 2017, [Online]. Available: https://sid.ir/paper/152079/en

Related Journal Papers