CLONING AND EXPRESSION OF THE NP GENE OF H9N2 AVIAN INFLUENZA VIRUS IN ESCHERCHIA COLI

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JAYDARI A.; SEYFI ABAD SHAPOURI M.R.; GHOURBANPOUR NAJAFABADI M.

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Journal: JOURNAL OF VETERINARY MICROBIOLOGY Year:2018 | Volume:14 | Issue:1 (36) Page(s): 81-88

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Title

CLONING AND EXPRESSION OF THE NP GENE OF H9N2 AVIAN INFLUENZA VIRUS IN ESCHERCHIA COLI

Author(s)

JAYDARI A. | SEYFI ABAD SHAPOURI M.R. | GHOURBANPOUR NAJAFABADI M. | Issue Writer Certificate

Keywords

CLONINGQ1

AVIAN INFLUENZAQ2

H9N2 SUB TYPEQ1

NP GENEQ1

Abstract

Avian influenza (AI) is a serious infectious disease with world-wide significance causing considerable financial losses in poultry industry. Serodiagnosis based on detection of antibody to the nucleoprotein (NP) that is conserved among all influenza A virus, can be used to demonstrate immunity against all subtypes of influenza A virus. The aim of this study was to produce recombinant nucleoprotein (NP). For this purpose, the coding region of nucleoprotein (NP) gene of A/chicken/Iran/AH-1/06 (H9N2) AI isolate was amplified by RTPCR and cloned into a prokaryotic expression vector (pMal-C2x) and transformed into E. coli BL21 (DE3). The purified recombinant vector for the expression of recombinant MBP-NP was used. Target protein with a weight of about 97 kDa by SDS-PAGE and subsequent Western blotting was evaluated. Observation the expressed protein with expected molecular weight in SDS-PAGE and positive reaction of this protein with bird, serum infected AVIAN INFLUENZA In western blot showed that the expression of NP GENE in E.coli was successful.

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APA: Copy

JAYDARI, A., SEYFI ABAD SHAPOURI, M.R., & GHOURBANPOUR NAJAFABADI, M.. (2018). CLONING AND EXPRESSION OF THE NP GENE OF H9N2 AVIAN INFLUENZA VIRUS IN ESCHERCHIA COLI. JOURNAL OF VETERINARY MICROBIOLOGY, 14(1 (36) ), 81-88. SID. https://sid.ir/paper/152177/en

Vancouver: Copy

JAYDARI A., SEYFI ABAD SHAPOURI M.R., GHOURBANPOUR NAJAFABADI M.. CLONING AND EXPRESSION OF THE NP GENE OF H9N2 AVIAN INFLUENZA VIRUS IN ESCHERCHIA COLI. JOURNAL OF VETERINARY MICROBIOLOGY[Internet]. 2018;14(1 (36) ):81-88. Available from: https://sid.ir/paper/152177/en

IEEE: Copy

A. JAYDARI, M.R. SEYFI ABAD SHAPOURI, and M. GHOURBANPOUR NAJAFABADI, “CLONING AND EXPRESSION OF THE NP GENE OF H9N2 AVIAN INFLUENZA VIRUS IN ESCHERCHIA COLI,” JOURNAL OF VETERINARY MICROBIOLOGY, vol. 14, no. 1 (36) , pp. 81–88, 2018, [Online]. Available: https://sid.ir/paper/152177/en

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