MOLECULAR DIAGNOSIS AND DETERMINATION OF AMINO ACID SEQUENCE OF HEMAGGLUTININ CLEAVAGE SITE OF AVIAN INFLUENZA VIRUSES ISOLATED FROM BROILERS IN NORTH KHORASAN PROVINCE

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BAHARI P.; POURBAKHSH S.A.; SHOUSHTARI A.A.H.; BAHMANINEJAD M.A.

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Paper Information

Journal: JOURNAL OF VETERINARY MICROBIOLOGY Year:2013 | Volume:9 | Issue:1 (26) Page(s): 61-72

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Title

MOLECULAR DIAGNOSIS AND DETERMINATION OF AMINO ACID SEQUENCE OF HEMAGGLUTININ CLEAVAGE SITE OF AVIAN INFLUENZA VIRUSES ISOLATED FROM BROILERS IN NORTH KHORASAN PROVINCE

Author(s)

BAHARI P. | POURBAKHSH S.A. | SHOUSHTARI A.A.H. | BAHMANINEJAD M.A. | Issue Writer Certificate

Keywords

AVIAN INFLUENZAQ2

RT-PCRQ2

H9-H7-H5 SUBTYPEQ2

CLEAVAGE SITE OF HEMAGGLUTININQ2

NORTH KHORASANQ2

Abstract

AVIAN INFLUENZA virus has been recognized as one of the most important pathogen in poultry that caused economic losses to the poultry industry of Iran. Rapid detection and pathotyping is the important priority in order to prevent extension of disease in Iran. RT-PCR method was used for the rapid detection of influenza viruses. The amino acid sequence of CLEAVAGE SITE OF HEMAGGLUTININ was considered as a molecular determinant for the prediction of pathogenicity influenza viruses. In this study, 160 tracheal swabs samples were taken from 20 poultry farm to monitor influenza infection in NORTH KHORASAN province. RT-PCR assay was developed based on the most common genotype in Iran detecting type A and H9, H5 and H7 subtypes by specific primers targeting Matrix (M) and Hemagglutinin(HA) genes, respectively. The samples which were positive as type A influenza inoculated to embryonated eggs of 9-10 days. After 3-5 days, the allantoic fluids were tested with Hemagglutinin assay. The samples that contained HA activity were inactivated with formalin 0.1%. These fluids were used for RNA extraction. A segment of 432bp was amplified by RT-PCR and nucleotide sequencing. The results showed that four poultry farms were positive for type A of influenza virus. All of them were negative for H5 or H7 but positive for H9 virus. The sequence analysis of hemagglutinin revealed that four isolates shared a KSSR motif at the cleavage site of HA. Although this motif represented low pathogenicity in poultry, it was different from previous Iranian isolates. As a result RT-PCR assay based on common genotype in region was considered as a rapid test for the detection of influenza viruses. The cleavage site sequence analyses indicated that these viruses have the potential for emerging highly pathogen influenza viruses. Therefore, continuous monitoring of viruses is being recommended.

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APA: Copy

BAHARI, P., POURBAKHSH, S.A., SHOUSHTARI, A.A.H., & BAHMANINEJAD, M.A.. (2013). MOLECULAR DIAGNOSIS AND DETERMINATION OF AMINO ACID SEQUENCE OF HEMAGGLUTININ CLEAVAGE SITE OF AVIAN INFLUENZA VIRUSES ISOLATED FROM BROILERS IN NORTH KHORASAN PROVINCE. JOURNAL OF VETERINARY MICROBIOLOGY, 9(1 (26)), 61-72. SID. https://sid.ir/paper/152195/en

Vancouver: Copy

BAHARI P., POURBAKHSH S.A., SHOUSHTARI A.A.H., BAHMANINEJAD M.A.. MOLECULAR DIAGNOSIS AND DETERMINATION OF AMINO ACID SEQUENCE OF HEMAGGLUTININ CLEAVAGE SITE OF AVIAN INFLUENZA VIRUSES ISOLATED FROM BROILERS IN NORTH KHORASAN PROVINCE. JOURNAL OF VETERINARY MICROBIOLOGY[Internet]. 2013;9(1 (26)):61-72. Available from: https://sid.ir/paper/152195/en

IEEE: Copy

P. BAHARI, S.A. POURBAKHSH, A.A.H. SHOUSHTARI, and M.A. BAHMANINEJAD, “MOLECULAR DIAGNOSIS AND DETERMINATION OF AMINO ACID SEQUENCE OF HEMAGGLUTININ CLEAVAGE SITE OF AVIAN INFLUENZA VIRUSES ISOLATED FROM BROILERS IN NORTH KHORASAN PROVINCE,” JOURNAL OF VETERINARY MICROBIOLOGY, vol. 9, no. 1 (26), pp. 61–72, 2013, [Online]. Available: https://sid.ir/paper/152195/en

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