IDENTIFICATION OF ESCHERICHIA COLI O157: H7 ON BOVINE CARCASSES IN RAFSANJAN BY USING CULTURE AND PCR METHOD

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SHAHROKHABADI R.; RAHIMI E.; MOMTAZ H.; POURSAHEBI R.

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Journal: JOURNAL OF VETERINARY MICROBIOLOGY Year:2012 | Volume:8 | Issue:1 (24) Page(s): 49-56

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Title

IDENTIFICATION OF ESCHERICHIA COLI O157: H7 ON BOVINE CARCASSES IN RAFSANJAN BY USING CULTURE AND PCR METHOD

Author(s)

SHAHROKHABADI R. | RAHIMI E. | MOMTAZ H. | POURSAHEBI R. | Issue Writer Certificate

Keywords

ESCHERICHIA COLI O157:H7Q2

BOVINE MEATQ2

PCRQ2

Abstract

ESCHERICHIA COLI O157:H7 is now recognized as an important cause of diarrhea, hemorrhagia colitis and hemolytic-uremic syndrome worldwide. Meat contaminated with animal feces is probably the major source of the E. coli O157:H7. In this study, we investigated the prevalence of E. coli O157:H7 in meat samples of bovine in Rafsanjan, Iran. This was a descriptive - analytical and descriptive study, which was conducted from April 2011 to February 2012. A total of 148 bovine carcasses were sampled by surface section of neck meat taken immediately after slaughter analyzed using microbiological examinations. Suspected colonies to E. coli O157:H7 were confirmed by a specific polymerase chain reaction method (PCR). The results showed that the contamination rate of samples to E. coli and E. coli O157:H7 were 36 (24.32%) and 10 (6.75%), respectively, six samples (4.05%) were identified as E. coli O157:H7, using polymerase chain reaction. Seasonal distribution showed that the highest prevalence of E. coli and E. coli O157:H7 occurred in summer samples (P<0.05).These results indicate that bovine can be a reservoir for E. coli O157:H7 and BOVINE MEAT may serve as a vehicle for the pathogen transmission to human.

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APA: Copy

SHAHROKHABADI, R., RAHIMI, E., MOMTAZ, H., & POURSAHEBI, R.. (2012). IDENTIFICATION OF ESCHERICHIA COLI O157: H7 ON BOVINE CARCASSES IN RAFSANJAN BY USING CULTURE AND PCR METHOD. JOURNAL OF VETERINARY MICROBIOLOGY, 8(1 (24)), 49-56. SID. https://sid.ir/paper/152203/en

Vancouver: Copy

SHAHROKHABADI R., RAHIMI E., MOMTAZ H., POURSAHEBI R.. IDENTIFICATION OF ESCHERICHIA COLI O157: H7 ON BOVINE CARCASSES IN RAFSANJAN BY USING CULTURE AND PCR METHOD. JOURNAL OF VETERINARY MICROBIOLOGY[Internet]. 2012;8(1 (24)):49-56. Available from: https://sid.ir/paper/152203/en

IEEE: Copy

R. SHAHROKHABADI, E. RAHIMI, H. MOMTAZ, and R. POURSAHEBI, “IDENTIFICATION OF ESCHERICHIA COLI O157: H7 ON BOVINE CARCASSES IN RAFSANJAN BY USING CULTURE AND PCR METHOD,” JOURNAL OF VETERINARY MICROBIOLOGY, vol. 8, no. 1 (24), pp. 49–56, 2012, [Online]. Available: https://sid.ir/paper/152203/en

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