Molecular phylogeny of the A genome using internal transcribed sequences (ITS) of ribosomal genes in some Triticum L. species

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SAFARI ZEINAB; MEHRABI ALI ASHRAF

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Journal: Iranian Journal of Field Crop Science Year:2017 | Volume:48 | Issue:2 Page(s): 483-491

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Information Journal Paper

Title

Molecular phylogeny of the A genome using internal transcribed sequences (ITS) of ribosomal genes in some Triticum L. species

Author(s)

SAFARI ZEINAB | MEHRABI ALI ASHRAF | Issue Writer Certificate

Keywords

A genomeQ1

dendrogramQ1

molecular phylogenyQ1

ribosomal DNAQ1

Abstract

The genus Triticum L. includes bread wheat and other important cultivated species, which are economically important for large parts of the human food. In this study, we conducted a phylogenetic analysis of A genome-possessing species of genus Triticum L. (T. aestivum, T. turgidum, T. urartu and T. boeticum). Here, the internal transcribed sequences (ITS) of nuclear ribosomal DNA were amplified by two pairs of primers in 26 genotypes from the above species. Sequenced amplicons were aligned by ClustalW. Divergence matrices and phylogenic dendrogram were made by MEGA 5. 0. Results revealed the full length of sequences of ITS1 and ITS2 were 650 bp and 700bp, respectively and G+C content were 60. 25 and 60. 50 in them. High levels of conservation in sequences were found among genotypes (%63 and %88). Phylogenetic analysis using amplified sequences were successfully divided diploid and polyploid wheats into individual groups. Regarding to the results, there were close relationships within T. aestivum and T. turgidum and also within T. urartu and T. boeticum. However, our analysis suggests that the ITS molecular markers seem to be proper tools for plant phylogenetic studies.

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APA: Copy

SAFARI, ZEINAB, & MEHRABI, ALI ASHRAF. (2017). Molecular phylogeny of the A genome using internal transcribed sequences (ITS) of ribosomal genes in some Triticum L. species. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 48(2 ), 483-491. SID. https://sid.ir/paper/153411/en

Vancouver: Copy

SAFARI ZEINAB, MEHRABI ALI ASHRAF. Molecular phylogeny of the A genome using internal transcribed sequences (ITS) of ribosomal genes in some Triticum L. species. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2017;48(2 ):483-491. Available from: https://sid.ir/paper/153411/en

IEEE: Copy

ZEINAB SAFARI, and ALI ASHRAF MEHRABI, “Molecular phylogeny of the A genome using internal transcribed sequences (ITS) of ribosomal genes in some Triticum L. species,” IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 48, no. 2 , pp. 483–491, 2017, [Online]. Available: https://sid.ir/paper/153411/en

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