ANALYSIS OF CAMV35S PROMOTER ACTIVITY IN DIFFERENT TISSUES OF TRANSGENIC TOBACCO PLANTS

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ABBASI A.; ABOOEI MEHRIZI F.; FOOL L.; SONOALL O.

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Journal: Iranian Journal of Field Crop Science Year:2010 | Volume:41 | Issue:4 Page(s): 803-810

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Title

ANALYSIS OF CAMV35S PROMOTER ACTIVITY IN DIFFERENT TISSUES OF TRANSGENIC TOBACCO PLANTS

Author(s)

ABBASI A. | ABOOEI MEHRIZI F. | FOOL L. | SONOALL O. | Issue Writer Certificate

Keywords

CAMV35S PROMOTERQ3

CONSTITUTIVE PROMOTERQ1

RNAIQ3

MATURE SEEDQ3

TOBACCO PLANTQ3

TRANSGENIC PLANTQ3

GENE EXPRESSIONQ3

Abstract

Each gene consists of three major parts namely promoter, the main body of the gene and terminator. Without the promoter, there whould be no GENE EXPRESSION. In general, promoter is specific for each gene, and leads to the regulation of the final product of the gene. One of the most common promoters employed by plant genetic engineer is CaMV35S. Although it is assumed that CaMV35S is a CONSTITUTIVE PROMOTER, but some reports suggest that this promoter is not expressed in all tissues and cell types. In addition, full accessible information regarding the expression profile of this promoter in all cells, in different tissues, and at the initial stages of development is lacking.Considering the above mentioned limitations, it leads one to study in more detail the CaMV35Spromoter activity. For an analysis of the promoter activity, tobacco TRANSGENIC PLANTs in which one of the interfering committed steps in their vitamin E biosynthesis had been inhibited through RNAI system were employed. Silencing of the gene involved in this interfering step resulted in reduced content of vitamin E in the TRANSGENIC PLANT as compared with the wild type. Since as a whole, vitamin E content will undergo reduction in TRANSGENIC PLANTs, the vitamin’s content in such different tissues as leaves, root, flower organs, immature and MATURE SEEDs of tobacco transgenic vs. wild type plants was measured. The data recorded here demonstrate thatCAMV35S PROMOTER has highly expressed activity in leaves and flower organ, moderate expression activity in roots and seeds, and while very lowly expressed in MATURE SEEDs.

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APA: Copy

ABBASI, A., ABOOEI MEHRIZI, F., FOOL, L., & SONOALL, O.. (2010). ANALYSIS OF CAMV35S PROMOTER ACTIVITY IN DIFFERENT TISSUES OF TRANSGENIC TOBACCO PLANTS. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 41(4), 803-810. SID. https://sid.ir/paper/153454/en

Vancouver: Copy

ABBASI A., ABOOEI MEHRIZI F., FOOL L., SONOALL O.. ANALYSIS OF CAMV35S PROMOTER ACTIVITY IN DIFFERENT TISSUES OF TRANSGENIC TOBACCO PLANTS. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2010;41(4):803-810. Available from: https://sid.ir/paper/153454/en

IEEE: Copy

A. ABBASI, F. ABOOEI MEHRIZI, L. FOOL, and O. SONOALL, “ANALYSIS OF CAMV35S PROMOTER ACTIVITY IN DIFFERENT TISSUES OF TRANSGENIC TOBACCO PLANTS,” IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 41, no. 4, pp. 803–810, 2010, [Online]. Available: https://sid.ir/paper/153454/en

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