DESIGNING AND DEVELOPMENT OF GAMMA INTERFERON CONSTRUCT AND EXPRESSION OF THIS PROTEININ BRASSICA NAPUS SEEDS

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BAGHERI KH.; JALALI JAVARAN M.; MAHBOUDI F.; MOEINI A.; ZEBARJADI A.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2010 | Volume:23 | Issue:2 Page(s): 151-160

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Title

DESIGNING AND DEVELOPMENT OF GAMMA INTERFERON CONSTRUCT AND EXPRESSION OF THIS PROTEININ BRASSICA NAPUS SEEDS

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Keywords

SEED SPECIFIC PROMOTER (NAPIN)Q3

KDEL RETENTION SIGNALQ3

RECOMBINANT PROTEINQ2

HUMAN GAMMA INTERFERONQ3

BRASSICA NAPUSQ3

Abstract

Some important Strategies to increase RECOMBINANT PROTEIN yield in plants include expression of protein in suitable plant tissue and improvement of protein stability and accumulation by targeting into subcellular organs using specific targeting signals. The ER contain different molecular chaperons and isomerase for folding of nascent proteins, which can prevent aggregation and formation of incorrect three dimensional structure and promote correct protein folding leading to RECOMBINANT PROTEIN stability and accumulation. In this perspective, seed-based platforms are particularly attractive because they allow RECOMBINANT PROTEINs to stably accumulate at a relatively high concentration in a compact biomass, which is beneficial for extraction and downstream processing. In this research for HUMAN GAMMA INTERFERON expression in BRASSICA NAPUS seeds and targeting into ER, we used seed specific promoter (Napin) and C-terminal KDEL sequence (ER retention signal). The fragment was cloned into pGEMÒ-T Easy vector and then was confirmed by PCR, restriction enzyme analysis and sequencing.The authentic PCR fragment was subcloned into a plant binary vector (pBI121). This construct cassette (pBI IFNg) was transferred to A. tumefaciens LBA4404 and then used to transform B. napus using an Agrobacterium-mediated petiole cotyledonary transformation. The transformed plants were screened on antibiotic-contained media The presence and expression of the transgene was confirmed in the transformants by PCR and SDS-PAGE. ELISA test was used for biological activity.

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APA: Copy

BAGHERI, KH., JALALI JAVARAN, M., MAHBOUDI, F., MOEINI, A., & ZEBARJADI, A.. (2010). DESIGNING AND DEVELOPMENT OF GAMMA INTERFERON CONSTRUCT AND EXPRESSION OF THIS PROTEININ BRASSICA NAPUS SEEDS. IRANIAN JOURNAL OF BIOLOGY, 23(2), 151-160. SID. https://sid.ir/paper/21336/en

Vancouver: Copy

BAGHERI KH., JALALI JAVARAN M., MAHBOUDI F., MOEINI A., ZEBARJADI A.. DESIGNING AND DEVELOPMENT OF GAMMA INTERFERON CONSTRUCT AND EXPRESSION OF THIS PROTEININ BRASSICA NAPUS SEEDS. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2010;23(2):151-160. Available from: https://sid.ir/paper/21336/en

IEEE: Copy

KH. BAGHERI, M. JALALI JAVARAN, F. MAHBOUDI, A. MOEINI, and A. ZEBARJADI, “DESIGNING AND DEVELOPMENT OF GAMMA INTERFERON CONSTRUCT AND EXPRESSION OF THIS PROTEININ BRASSICA NAPUS SEEDS,” IRANIAN JOURNAL OF BIOLOGY, vol. 23, no. 2, pp. 151–160, 2010, [Online]. Available: https://sid.ir/paper/21336/en

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