IN VITRO REGENERATION OF SAINFOIN (ONOBRYCHIS SATIVA) VIA SHOOT APEX EXPLANT

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HONARMAND LEYLI; ZARE NASSER; ASGHARI ZAKARIA RASOOL; SHEIKHZADE MOSADEGH PARISA

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Journal: Iranian Journal of Field Crop Science Year:2016 | Volume:47 | Issue:2 Page(s): 315-328

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Information Journal Paper

Title

IN VITRO REGENERATION OF SAINFOIN (ONOBRYCHIS SATIVA) VIA SHOOT APEX EXPLANT

Author(s)

HONARMAND LEYLI | ZARE NASSER | ASGHARI ZAKARIA RASOOL | SHEIKHZADE MOSADEGH PARISA | Issue Writer Certificate

Keywords

VITRO PROPAGATIONQ1

ONOBRYCHIS SATIVAQ3

TISSUE CULTUREQ1

Abstract

The growing demand for animal products has made importance of the utilization of biotechnological techniques in improvement of forage crops such as sainfoin. In this research, the effects of different levels of auxin (NAA and IBA) and cytokinin (BAP, Zeatin and TDZ) on in vitro growth response of shoot apex explants of Shahrkord and Khansar cultivars of sainfoin were investigated. For this, shoot apex explants were prepared from 4-7 days old seedling grown and cultured on solidified MS medium containing different levels of auxin and cytokinin. The results indicated that the percentage of multiple shoots, number of shoots per explants and shoot length were significantly influenced by cultivar, plant growth regulators combination and its interaction. The percentage of multiple shoots and number of shoots per explants were varied from 0 to 89.7% and 1 to 5.03, respectively. Generally, the percentage of callus induction, multiple shoots and number of shoots per explant in the Khansar cultivar was significantly higher than those of Shahrkord cultivar. The highest percentage of shoots (98.15%), multiple shoots (89.17%) and number of shoots per explant (4.3) were obtained in the Khansar cultivar with MS medium containing 0.1 mgL-1 IBA and 3 mgL-1 BAP. Root induction and appropriate growth of roots were observed on MS medium without plant growth regulators. These results are highly valuable for in vitro culture and micropropagation of sainfoin as well as in breeding programs and transformation of this plant.

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APA: Copy

HONARMAND, LEYLI, ZARE, NASSER, ASGHARI ZAKARIA, RASOOL, & SHEIKHZADE MOSADEGH, PARISA. (2016). IN VITRO REGENERATION OF SAINFOIN (ONOBRYCHIS SATIVA) VIA SHOOT APEX EXPLANT. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 47(2), 315-328. SID. https://sid.ir/paper/153783/en

Vancouver: Copy

HONARMAND LEYLI, ZARE NASSER, ASGHARI ZAKARIA RASOOL, SHEIKHZADE MOSADEGH PARISA. IN VITRO REGENERATION OF SAINFOIN (ONOBRYCHIS SATIVA) VIA SHOOT APEX EXPLANT. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2016;47(2):315-328. Available from: https://sid.ir/paper/153783/en

IEEE: Copy

LEYLI HONARMAND, NASSER ZARE, RASOOL ASGHARI ZAKARIA, and PARISA SHEIKHZADE MOSADEGH, “IN VITRO REGENERATION OF SAINFOIN (ONOBRYCHIS SATIVA) VIA SHOOT APEX EXPLANT,” IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 47, no. 2, pp. 315–328, 2016, [Online]. Available: https://sid.ir/paper/153783/en

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