OPTIMIZATION OF DIRECT AND CALLUS MEDIATED REGENERATION OF JATROPHA CURCUS THROUGH IN VITRO ORGANOGENESIS

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FAKHRODINNEJAD F.; AGHDASI M.; MASHAYEKHI K.; KHALAFI M.

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Journal: Journal of Plant Production Research Year:2015 | Volume:21 | Issue:4 Page(s): 117-134

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Title

OPTIMIZATION OF DIRECT AND CALLUS MEDIATED REGENERATION OF JATROPHA CURCUS THROUGH IN VITRO ORGANOGENESIS

Author(s)

FAKHRODINNEJAD F. | AGHDASI M. | MASHAYEKHI K. | KHALAFI M. | Issue Writer Certificate

Keywords

CALLUSQ2

JATROPHA CURCASQ2

ORGANOGENESISQ2

PLANT GROWTH REGULATORQ2

TISSUE CULTUREQ2

Abstract

The JATROPHA CURCAS L., a plant belongs to Euphorbiaceae family, has great economic and medicinally value. There is high amount of oil in seeds of this plant. The seed oil content varies from 4 to 40%, which is used in biodiesel, candle, varnish and lubrication production. Because of low germination ability of seed, propagation of JATROPHA CURCAS via seed is not usual. At this time, plant TISSUE CULTURE provide an alternative approach to the plants which are difficult to propagate, or has a long propagation period. The purpose of this study was to optimize JATROPHA CURCAS TISSUE CULTURE for CALLUS formation and propagation. All experiments were performed in complete randomized design with nine replications. In the present study, the effects of different concentrations of 6-benzyladenine (BA) and indol-3-butyric acid (IBA) hormones (0, 0.25, 0.5, 1, 1.5, 2, 3 mg/L) were investigated on CALLUS formation of leaf, petiole and stem explants in MS medium. Also combination of different concentrations of 6-benzyladenine (BA) (0, 0.5, 1, 2, 3 mg/L) and indol-3- butyric acid (IBA) (0, 0.25, 0.5, 1, 1.5, 2, 3 mg/L) was investigated on shoot induction in node explants. In the next step, obtained shoot were grown on MS medium containing 0, 0.5, 1, 1.5 and 2 mg/L of IBA to induce root formation. The results showed that the combination of BA and IBA is more effective for CALLUS formation. The highest percentage of CALLUS formation was observed at 2 mg/L BA and 1 mg/L IBA in stem explants. The results showed that the highest percentage of shoot induction was observed at 0.5 mg/L BA and 0.25 mg/L IBA in node explants. Also, maximum regenerated root was observed in MS medium supplemented with 0.5 or 1 mg/L of IBA treatment. Finally, the obtained plants were transferred into soil medium. The shoot bud induction along with CALLUS formation was observed in petiole explants (64%) at 1.5 mg/L BA and 1 mg/L IBA and Leaf explants (56%) at 3 mg/L BA and 0.5 mg/L IBA hormones.

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APA: Copy

FAKHRODINNEJAD, F., AGHDASI, M., MASHAYEKHI, K., & KHALAFI, M.. (2015). OPTIMIZATION OF DIRECT AND CALLUS MEDIATED REGENERATION OF JATROPHA CURCUS THROUGH IN VITRO ORGANOGENESIS. JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES), 21(4), 117-134. SID. https://sid.ir/paper/155931/en

Vancouver: Copy

FAKHRODINNEJAD F., AGHDASI M., MASHAYEKHI K., KHALAFI M.. OPTIMIZATION OF DIRECT AND CALLUS MEDIATED REGENERATION OF JATROPHA CURCUS THROUGH IN VITRO ORGANOGENESIS. JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES)[Internet]. 2015;21(4):117-134. Available from: https://sid.ir/paper/155931/en

IEEE: Copy

F. FAKHRODINNEJAD, M. AGHDASI, K. MASHAYEKHI, and M. KHALAFI, “OPTIMIZATION OF DIRECT AND CALLUS MEDIATED REGENERATION OF JATROPHA CURCUS THROUGH IN VITRO ORGANOGENESIS,” JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES), vol. 21, no. 4, pp. 117–134, 2015, [Online]. Available: https://sid.ir/paper/155931/en

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