PRODUCTION OF COMMON YEW (TAXUS BACCATA L.) SEEDLINGS BY EMBRYO CULTURE UNDER IN VITRO CONDITIONS

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RAZAVI S.A.; HOSSEINI NASR S.M.; ROSTAMI CHRATI F.; REZADOOST H.

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Journal: Journal of Wood and Forest Science and Technology Year:2016 | Volume:23 | Issue:SUPPLEMENT 1 Page(s): 115-133

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Title

PRODUCTION OF COMMON YEW (TAXUS BACCATA L.) SEEDLINGS BY EMBRYO CULTURE UNDER IN VITRO CONDITIONS

Author(s)

RAZAVI S.A. | HOSSEINI NASR S.M. | ROSTAMI CHRATI F. | REZADOOST H. | Issue Writer Certificate

Keywords

TAXUS BACCATA L.Q2

EMBRYOQ1

IN VITRO CULTUREQ2

COLD STRATIFICATIONQ1

Abstract

Background and objectives: TAXUS BACCATA L. is one of the rare native coniferous species of Iran which its distribution is from Astara to Ali Abad Katoul (Golestan province) and Arasbaran forests. This species is highly valuable for preservation of genetic resources, medicinal uses and etc. At present, it is an endangered species for long seed dormancy, slow growth, illegal utilization and other disturbance factors. In order to conservation of present sites and their extension, production of yew seedlings by EMBRYO culture underin vitro conditions was considered.Materials and methods: Seeds after collection from the natural site in Afra Takhteh region, divided into 2 mature and immature groups. Then seeds stratified in sand layers and low temperature for 9 months. Seeds were soaked for 48 hours in running tap water and then disinfected. In order to sterilization of seeds, 7 treatments were considered. In this study, the EMBRYO of sterilized seeds split from endosperm and was cultured in Murashige and Skoog (MS) medium that it was enriched with ascorbic acid (50, 100 mg L-1), casein hydrolysate (50, 100 mg L-1), yeast extraction (0.5, 1 g L-1) and activated charcoal (2%). For slow growth of yew seedlings, providing of nutrients and medium, all the grown EMBRYOs were sub cultured biweekly. Data were analyzed with one and two ways ANOVA and Duncan tests.Results: The results show that the best method for seed sterilization was H2O2 10% (for 15 min), and ethanol 70% (for 1 min) that any infection was observed. Other results show that the EMBRYOs started to regeneration about 10 days. The maximum of regeneration and normal seedlings was belonged to treatment 1 that it was consisted of mature seed, ascorbic acid and casein hydrolysate (100 mg L-1), yeast extraction (1 g L-1) and activated charcoal (2%).Conclusion: MS medium containing 100 mg L-1 ascorbic acid and casein hydrolysate, 1 g L-1 yeast extraction and 2% activated charcoal, that the EMBRYO of mature seeds had suitable regeneration, is suggested as suitable protocol for production of TAXUS BACCATA L. seedlings.

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APA: Copy

RAZAVI, S.A., HOSSEINI NASR, S.M., ROSTAMI CHRATI, F., & REZADOOST, H.. (2016). PRODUCTION OF COMMON YEW (TAXUS BACCATA L.) SEEDLINGS BY EMBRYO CULTURE UNDER IN VITRO CONDITIONS. JOURNAL OF WOOD AND FOREST SCIENCE AND TECHNOLOGY, 23(SUPPLEMENT 1), 115-133. SID. https://sid.ir/paper/156836/en

Vancouver: Copy

RAZAVI S.A., HOSSEINI NASR S.M., ROSTAMI CHRATI F., REZADOOST H.. PRODUCTION OF COMMON YEW (TAXUS BACCATA L.) SEEDLINGS BY EMBRYO CULTURE UNDER IN VITRO CONDITIONS. JOURNAL OF WOOD AND FOREST SCIENCE AND TECHNOLOGY[Internet]. 2016;23(SUPPLEMENT 1):115-133. Available from: https://sid.ir/paper/156836/en

IEEE: Copy

S.A. RAZAVI, S.M. HOSSEINI NASR, F. ROSTAMI CHRATI, and H. REZADOOST, “PRODUCTION OF COMMON YEW (TAXUS BACCATA L.) SEEDLINGS BY EMBRYO CULTURE UNDER IN VITRO CONDITIONS,” JOURNAL OF WOOD AND FOREST SCIENCE AND TECHNOLOGY, vol. 23, no. SUPPLEMENT 1, pp. 115–133, 2016, [Online]. Available: https://sid.ir/paper/156836/en

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