ANOVEL CHEMICAL METHOD FOR MEASURING KETONE BODIES IN SUBCLINICAL KETOSIS

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GHOLAMPOUR H.; ASADIAN P.; ERFANMANESH A.; JAHANTIGH M.

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Journal: Iranian Journal of Veterinary Medicine Year:2012 | Volume:6 | Issue:1 Page(s): 7-11

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Information Journal Paper

Title

ANOVEL CHEMICAL METHOD FOR MEASURING KETONE BODIES IN SUBCLINICAL KETOSIS

Author(s)

GHOLAMPOUR H. | ASADIAN P. | ERFANMANESH A. | JAHANTIGH M. | Issue Writer Certificate

Keywords

KETOSISQ2

BETAHYDROXYBUTYRIC ACIDQ2

ACETOACETATEQ2

NANOSILVERQ2

Abstract

BACKGROUNDS: Subclinical KETOSIS can cause greater economic loss due to a lack of clinical symptoms.OBJECTIVES: The present study was aimed to design a chemical method for measuring serum ketone bodies in the affected subclinical cows.METHODS: ACETOACETATE concentrations were measured using a nitroprusside reaction and b-hydroxy butyric acid (BHBA) which was oxidized to ACETOACETATE using NANOSILVER particles to determine its concentration. Recovery tests were done for different concentrations of betahydroxy butyrate in bovine pooled serum.RESULTS: Actoacetate levels were in range of 0.1-6 mM, and the values for BHBA were found to be in the range of 0.125-3 mM.CONCLUSIONS: It was concluded that this technique can be considered as a simple method for measuring ketone bodies in biological fluids.

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APA: Copy

GHOLAMPOUR, H., ASADIAN, P., ERFANMANESH, A., & JAHANTIGH, M.. (2012). ANOVEL CHEMICAL METHOD FOR MEASURING KETONE BODIES IN SUBCLINICAL KETOSIS. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 6(1), 7-11. SID. https://sid.ir/paper/157938/en

Vancouver: Copy

GHOLAMPOUR H., ASADIAN P., ERFANMANESH A., JAHANTIGH M.. ANOVEL CHEMICAL METHOD FOR MEASURING KETONE BODIES IN SUBCLINICAL KETOSIS. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2012;6(1):7-11. Available from: https://sid.ir/paper/157938/en

IEEE: Copy

H. GHOLAMPOUR, P. ASADIAN, A. ERFANMANESH, and M. JAHANTIGH, “ANOVEL CHEMICAL METHOD FOR MEASURING KETONE BODIES IN SUBCLINICAL KETOSIS,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 6, no. 1, pp. 7–11, 2012, [Online]. Available: https://sid.ir/paper/157938/en

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