Evaluation of the Hydroxyproline Content in the Frozen Allograft Tendon Impregnated With the Mesenchymal Cells & PRP in Lamb

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Naeem Al Khalifah Rafid Majeed; SHARIFI DAVOOD; BOKAEI SAEED; BASHIRI ALIREZA; SHAD HOSSEIN; Esanejad Rouhoulah; SHAMS GHOLAM REZA

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Journal: Iranian Journal of Veterinary Medicine Year:2019 | Volume:13 | Issue:1 Page(s): 1-9

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Title

Evaluation of the Hydroxyproline Content in the Frozen Allograft Tendon Impregnated With the Mesenchymal Cells & PRP in Lamb

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Keywords

Frozen tendonQ1

HydroxyprolineQ1

LambQ1

Mesenchymal cellQ1

PRPQ1

Abstract

BACKGROUND: Tendon grafting is mostly required to repair an injury to flexor tendon and due to the importance of this tendon; it needs methods to speed up the re-vitality of the tendon allograft. OBJECTIVES: The aim was to investigate the efficacy of the Mesenchymal cells and platelet rich plasma on the Hydroxyproline content of frozen allograft tendons after grafting in Lamb. METHODS: In this experimental study, mid portion (five cm) of SDFT of fifteen Lambs, from both forelimbs were removed and replaced with frozen allografts tendon. Animals were divided into 3 subgroups of 5 Lambs each: control, PRP and MSCs. The Hydroxyproline concentration was measured by after 60 days. Data was analyzed using student t test at P<0. 05%. RESULTS: The normal mean values of Hydroxyproline content was 137. 171± 5. 291 mg/g dry matter which 87. 694± 6. 502 in control group but 99. 694± 1. 839 in PRP group and in group treated with MSC was recorded 134. 322± 2. 123 mg/g dry matter (P<0. 05%) it was quite significantly different between control tendons with that of normal one of the same animal. There was marked increase in Hydroxyproline content of MSC group when compare with that of control and PRP groups. CONCLUSIONS: The results of this study showed that MSCs could enhance HP content in the frozen grafted tendon in Lamb.

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APA: Copy

Naeem Al Khalifah, Rafid Majeed, SHARIFI, DAVOOD, BOKAEI, SAEED, BASHIRI, ALIREZA, SHAD, HOSSEIN, Esanejad, Rouhoulah, & SHAMS, GHOLAM REZA. (2019). Evaluation of the Hydroxyproline Content in the Frozen Allograft Tendon Impregnated With the Mesenchymal Cells & PRP in Lamb. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 13(1 ), 1-9. SID. https://sid.ir/paper/158043/en

Vancouver: Copy

Naeem Al Khalifah Rafid Majeed, SHARIFI DAVOOD, BOKAEI SAEED, BASHIRI ALIREZA, SHAD HOSSEIN, Esanejad Rouhoulah, SHAMS GHOLAM REZA. Evaluation of the Hydroxyproline Content in the Frozen Allograft Tendon Impregnated With the Mesenchymal Cells & PRP in Lamb. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2019;13(1 ):1-9. Available from: https://sid.ir/paper/158043/en

IEEE: Copy

Rafid Majeed Naeem Al Khalifah, DAVOOD SHARIFI, SAEED BOKAEI, ALIREZA BASHIRI, HOSSEIN SHAD, Rouhoulah Esanejad, and GHOLAM REZA SHAMS, “Evaluation of the Hydroxyproline Content in the Frozen Allograft Tendon Impregnated With the Mesenchymal Cells & PRP in Lamb,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 13, no. 1 , pp. 1–9, 2019, [Online]. Available: https://sid.ir/paper/158043/en

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