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Information Journal Paper

Title

In vitro efficacy of the anti fungal activity of Thymus vulgaris and Origanum vulgare on Saprolegnia sp. from rainbow trout (Oncorhynchus mykiss) in propogation and breeding center of Shahid Motahari, Yasuj province

Pages

  11-18

Abstract

 Curret survey performed to examine the effect of anti fungal extract of Thymus vulgaris and Origanu majorana on Saprolegnia sp. In propogation of Shshid Motahhari in Yasuj province. For this purpose 50 fungal samples from fin, gill surface and skin of Rainbow trout were got randomly and cultured on sabouraud dextrose agar medium then was transferred to Veterinary Medicine of Islamic Azad University, Kazerun branch. Cultured samples in ancubator were diagnosed as Saprolegnia sp. by identification key and were cultured on Trypton soy broth once more. Extraction was done by method of suspension in methanol 96%. The effect of antfungal was examined by producing continuous concentration of extracts to determine MIC and MBC. The results showed that the minimum inhibitory concentration (MIC) of Thymus vulgaris extract at concentration of 40 percent, 50 percent, 60 percent, 70 percent and 80 percent, were 16, 16, 8, 4 and 2 mg/ml respectively and the minimum inhibitory concentration (MIC) of the Origanu majorana extract in concentrations of 40 percent, 50 percent, 60 percent, 70 percent and 80 percent, were 16, 8, 8, 4 and 2 mg/ml, respectively. The minimum fungicidal concentration (MFC) of Thymus vulgaris extract at concentration of 60 percent, 70 percent and 80 percent were 16, 4 and 4 mg/ml respectively and the minimum fungicidal concentration (MFC) of Origanu majoranaextract at concentration of 60 percent, 70 percent and 80 percent were 16, 8, 8 mg/ml respectively. According to Two-Way Analysis of Variance there was a significant relationship between extract concentration of herbal drug and their effects )P≥ 0. 05).

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    APA: Copy

    GOLCHIN MANSHADI, A.R., Jarakani Bayat, s., & TARAHOMI, M.. (2017). In vitro efficacy of the anti fungal activity of Thymus vulgaris and Origanum vulgare on Saprolegnia sp. from rainbow trout (Oncorhynchus mykiss) in propogation and breeding center of Shahid Motahari, Yasuj province. NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES), 10(4 ), 11-18. SID. https://sid.ir/paper/161505/en

    Vancouver: Copy

    GOLCHIN MANSHADI A.R., Jarakani Bayat s., TARAHOMI M.. In vitro efficacy of the anti fungal activity of Thymus vulgaris and Origanum vulgare on Saprolegnia sp. from rainbow trout (Oncorhynchus mykiss) in propogation and breeding center of Shahid Motahari, Yasuj province. NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES)[Internet]. 2017;10(4 ):11-18. Available from: https://sid.ir/paper/161505/en

    IEEE: Copy

    A.R. GOLCHIN MANSHADI, s. Jarakani Bayat, and M. TARAHOMI, “In vitro efficacy of the anti fungal activity of Thymus vulgaris and Origanum vulgare on Saprolegnia sp. from rainbow trout (Oncorhynchus mykiss) in propogation and breeding center of Shahid Motahari, Yasuj province,” NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES), vol. 10, no. 4 , pp. 11–18, 2017, [Online]. Available: https://sid.ir/paper/161505/en

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