SPERM QUALITY OF PERSIAN STURGEON (ACIPENSER PERSICUS) AFTER CRYOPRESERVATION WITH DIMETHYL SULFOXIDE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AHMADI FAKHABI M.; ZAMINI A.A.; BARADRAN NOVEYRI SH.; FAROKHROUZ M.; MOBARHANFARD A.R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES) Year:2009 | Volume:3 | Issue:2 Page(s): 63-72

Download Full-Text

Persian Verion

View:

1,154

Download:

Cites:

Information Journal Paper

Title

SPERM QUALITY OF PERSIAN STURGEON (ACIPENSER PERSICUS) AFTER CRYOPRESERVATION WITH DIMETHYL SULFOXIDE

Author(s)

Keywords

SPERMQ2

ACIPENSER PERSICUSQ2

MOTILITY RATEQ3

MOTILITY TIMEQ2

CRYOPRESERVATIONQ3

DMSOQ3

Abstract

The maximum ability of SPERM for fertilization is 5-6 hours after collecting SPERM from males in normal temperature. While by semen freezing and use of suitable extenders and cryoprotectants we can conserve it in liquid nitrogen for several years and we can use stored SPERMs in essential Conditions.Varied cryoprotectants are used in sturgeon semen CRYOPRESERVATION but DMSO is the most important cryoprotectant. In this study, we have investigated the protective effect of DMSO as a cryoprotectant using different concentrations (8%, 10%, 12%, and 15%) and we have determined SPERM MOTILITY RATE and its MOTILITY TIME after thaw. The SPERM of Persian sturgeon was collected from five males. They were diluted in appropriate extender containing Tris, Sacharose, egg yolk, and dimethyl sulfoxide in 1:1 ratio and the suspension were poured in to 0.5 ml straws and frozen in -196oC liquid nitrogen vapour in a three storage program. Then straws were transferred to liquid nitrogen tank. The SPERM samples were thawed in 40oC water bath for 25s. The SPERM MOTILITY RATE and its MOTILITY TIME were observed using microscope. According to the results, the SPERM MOTILITY RATE after preservation in liquid nitrogen about 7 days were measured as 8.5±0.61, 12.54±2.57, 19±5.66, and 16.3±6.15% in concentrations 8%, 10%, 12%, and 15%, the SPERM MOTILITY TIME after this time was 184.3±28.87, 267.3±36.54, 274±50.51, and 218±63.85s, respectively. Also the SPERM MOTILITY RATE after preservation in liquid nitrogen about 72 days were measured as 14.25±5.50, 10.50±1.61, 7.50±1.81, and 13.75±2.74%, the SPERM MOTILITY TIME after this time was 195±39.50, 194.50±43.63, 154±37.34, and 221.70±47.31s, respectively. There was no statistical significant difference between selected concentrations. However, 12 and 15% of DMSO had better MOTILITY RATE and time. Results indicated that the increase in concentration of DMSO from 8% to 15% does not change the percentage of motile cells and MOTILITY TIME on SPERM CRYOPRESERVATION of Persian sturgeon.

Cites

No record.

References

Cite

APA: Copy

AHMADI FAKHABI, M., ZAMINI, A.A., BARADRAN NOVEYRI, SH., FAROKHROUZ, M., & MOBARHANFARD, A.R.. (2009). SPERM QUALITY OF PERSIAN STURGEON (ACIPENSER PERSICUS) AFTER CRYOPRESERVATION WITH DIMETHYL SULFOXIDE. NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES), 3(2), 63-72. SID. https://sid.ir/paper/161654/en

Vancouver: Copy

AHMADI FAKHABI M., ZAMINI A.A., BARADRAN NOVEYRI SH., FAROKHROUZ M., MOBARHANFARD A.R.. SPERM QUALITY OF PERSIAN STURGEON (ACIPENSER PERSICUS) AFTER CRYOPRESERVATION WITH DIMETHYL SULFOXIDE. NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES)[Internet]. 2009;3(2):63-72. Available from: https://sid.ir/paper/161654/en

IEEE: Copy

M. AHMADI FAKHABI, A.A. ZAMINI, SH. BARADRAN NOVEYRI, M. FAROKHROUZ, and A.R. MOBARHANFARD, “SPERM QUALITY OF PERSIAN STURGEON (ACIPENSER PERSICUS) AFTER CRYOPRESERVATION WITH DIMETHYL SULFOXIDE,” NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES), vol. 3, no. 2, pp. 63–72, 2009, [Online]. Available: https://sid.ir/paper/161654/en

Related Journal Papers