SURVEY OF METHANOL EFFECT ON SPERM MOTILITY RATE AND SPERM MOTILITY TIME OF PERSIAN STURGEON (ACIPENSER PERSICUS) AFTER CRYOPRESERVATION

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AHMADI FAKHABI M.; ZAMINI A.A.; BARADRAN NOVEYRI SH.

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Journal: JOURNAL OF BIOLOGY SCIENCE Year:2009 | Volume:3 | Issue:3 (SERIES NO. 10) Page(s): 1-12

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Title

SURVEY OF METHANOL EFFECT ON SPERM MOTILITY RATE AND SPERM MOTILITY TIME OF PERSIAN STURGEON (ACIPENSER PERSICUS) AFTER CRYOPRESERVATION

Author(s)

AHMADI FAKHABI M. | ZAMINI A.A. | BARADRAN NOVEYRI SH. | Issue Writer Certificate

Keywords

ACIPENSER PERSICUSQ3

SPERMQ2

METHANOLQ3

CRYOPRESERVATIONQ3

MOTILITY RATEQ3

MOTILITY TIMEQ3

Abstract

One of the usages of SPERM CRYOPRESERVATION is help to protect of endangered species. The life of SPERMatozoa is very short time in natural conditions and we can not conserve it for long time. While by semen freezing and use of suitable extenders and cryoprotectants we can conserve it in liquid nitrogen for several years and we can use stored SPERMs in essential times. In this research milt obtained from five Persian sturgeon (ACIPENSER PERSICUS) were diluted using Tris-sacharose-egg yolk (23.4 mM Tris, 1.8mM sacharose, 20% egg yolk) as extender and METHANOL as cryoprotectant in four concentration (8, 10, 12, and 15%). The suspension was poured in to 0.5 ml straws and frozen in-196°C liquid nitrogen vapour in a three storage programe. Then straws were transferred to liquid nitrogen tank. The SPERM samples were thawed in a 40°C water bath for 25s and the SPERM MOTILITY RATE and it's MOTILITY TIME were observed using microscope. There was not statistical significant difference between selected concentration (P>0.05) but the highest post-thaw MOTILITY RATE and MOTILITY TIME (7.00±2.15% and 102.30±39.14s respectively) was found using 8% METHANOL after preservation in liquid nitrogen about 7 days while the lowest post-thaw MOTILITY RATE and MOTILITY TIME (1.25±0.56% and 23.00±9.60s respectively) was found using 8% METHANOL after preservation in liquid nitrogen about 72 days. Results indicate that the increase in SPERM preservation in liquid nitrogen has a negative effect on SPERM quality and also increase in concentration of METHANOL from 8% to 15% does not change the percentage of motile cells and MOTILITY TIME on SPERM CRYOPRESERVATION of Persian sturgeon. In This project METHANOL as a cryoprotectant had not successful efficiency on SPERM cryopresevation of Persian sturgeon.

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APA: Copy

AHMADI FAKHABI, M., ZAMINI, A.A., & BARADRAN NOVEYRI, SH.. (2009). SURVEY OF METHANOL EFFECT ON SPERM MOTILITY RATE AND SPERM MOTILITY TIME OF PERSIAN STURGEON (ACIPENSER PERSICUS) AFTER CRYOPRESERVATION. JOURNAL OF BIOLOGY SCIENCE, 3(3 (SERIES NO. 10)), 1-12. SID. https://sid.ir/paper/164201/en

Vancouver: Copy

AHMADI FAKHABI M., ZAMINI A.A., BARADRAN NOVEYRI SH.. SURVEY OF METHANOL EFFECT ON SPERM MOTILITY RATE AND SPERM MOTILITY TIME OF PERSIAN STURGEON (ACIPENSER PERSICUS) AFTER CRYOPRESERVATION. JOURNAL OF BIOLOGY SCIENCE[Internet]. 2009;3(3 (SERIES NO. 10)):1-12. Available from: https://sid.ir/paper/164201/en

IEEE: Copy

M. AHMADI FAKHABI, A.A. ZAMINI, and SH. BARADRAN NOVEYRI, “SURVEY OF METHANOL EFFECT ON SPERM MOTILITY RATE AND SPERM MOTILITY TIME OF PERSIAN STURGEON (ACIPENSER PERSICUS) AFTER CRYOPRESERVATION,” JOURNAL OF BIOLOGY SCIENCE, vol. 3, no. 3 (SERIES NO. 10), pp. 1–12, 2009, [Online]. Available: https://sid.ir/paper/164201/en

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