Bacterial study of caudal fin rot in brood stocks of Salmo trutta caspius by method of PCR/RFLP from the propagation and breeding center of Shahid Bahonar of Kelardasht region

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GOLCHIN MANSHADI A.R.

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Paper Information

Journal: NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES) Year:2016 | Volume:9 | Issue:4 Page(s): 71-78

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Title

Bacterial study of caudal fin rot in brood stocks of Salmo trutta caspius by method of PCR/RFLP from the propagation and breeding center of Shahid Bahonar of Kelardasht region

Author(s)

GOLCHIN MANSHADI A.R. | Issue Writer Certificate

Keywords

PseudomonasQ2

Salmo trutta caspiusQ2

PCRQ2

RFLPQ2

Abstract

Fin rot syndrome is one of the difficulties in the aquaculture industry, especially in brood stocks which many infectious and non-infectious causes are involved in its onset. Current survey was performed In order to study of the syndrom with emphasis on Pseudomonas in Salmo trutta caspius from the salmonids propagation and breeding center of Shahid Bahonar of kelardasht One hundred and eighty brood stocks having fin damage symptoms were chosen. Bacterial sample from each fish was cultured on Pseudomonas specific media. In the next stage bacterial samples purified and finally seventeen samples were chosen and purified for molecular examination including PCR (Polymerase Chain Reaction) test by using 16S rDNA gen and RFLP (Restriction Fragment Length Polymorphisms) technique with four restriction enzymes including AluI, HinfI, RsaI, and Tru1I. The result of gel electrophoresis of PCR production of Pseudomonas samples showed 990 pb after UV radiation and Gel electrophoresis of Pseudomonas samples using restriction enzymes showed at least five species or biovars after UV radiation. Several species of Pseudomonas genus involed in fin rot.

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APA: Copy

GOLCHIN MANSHADI, A.R.. (2016). Bacterial study of caudal fin rot in brood stocks of Salmo trutta caspius by method of PCR/RFLP from the propagation and breeding center of Shahid Bahonar of Kelardasht region. NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES), 9(4 ), 71-78. SID. https://sid.ir/paper/161778/en

Vancouver: Copy

GOLCHIN MANSHADI A.R.. Bacterial study of caudal fin rot in brood stocks of Salmo trutta caspius by method of PCR/RFLP from the propagation and breeding center of Shahid Bahonar of Kelardasht region. NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES)[Internet]. 2016;9(4 ):71-78. Available from: https://sid.ir/paper/161778/en

IEEE: Copy

A.R. GOLCHIN MANSHADI, “Bacterial study of caudal fin rot in brood stocks of Salmo trutta caspius by method of PCR/RFLP from the propagation and breeding center of Shahid Bahonar of Kelardasht region,” NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES), vol. 9, no. 4 , pp. 71–78, 2016, [Online]. Available: https://sid.ir/paper/161778/en

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