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Information Journal Paper

Title

POPULATION GENETIC STRUCTURE OF NEOGOBIUS CASPIUS (EICHWALD, 1831) IN THE SOUTH CASPIAN SEA USING PCR-RFLP MARKER

Pages

  21-26

Abstract

 Neogobius caspius is a small benthic fish that is native of the CASPIAN SEA. The importance of this fish is because of its role as a main food resource of the Sturgeon fish. The genetic diversity of N.caspius population in the CASPIAN SEA was studied using PCR-RFLP technique. A total of 135 samples of N.caspius were collected from costal line in the north CASPIAN SEA, including specimens from costal of Anzali and Chalus. Genomic DNA was extracted by phenol-chloroform method and then was amplified using a pair primer of cytochrom b gene, 2 tRNA gene and the control region sequences by a thermal cycler.R: D2(5'-CCGGAGTATGTAGGGCATTCTCAC-3')F: CY1(5'-YYTAACCRRGACYAATGACTTGA-3')12 restriction enzyme were used to digest the target gene region including: AluI - Alw26I(BsmAI) - BSeNI(BSRI)- DraI- MboI- RsaI- TasI- HincII- BsuRI(HaeIII) - Bsh1285I - Hin1I- Bsu15I. Digested PCR products were observed by silver staining method followed by polyacrilamid gel electrophoresis (PAGE). The results showed the same pattern among the species. There was no polymorphism and no differentiation in population in the NEOGOBIUS CASPIUS fish in the southern coast of CASPIAN SEA.

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    APA: Copy

    GHOTB RAZMJOUE, E., REZVANI, SOHRAB, GHAVAM MOSTAFAVI, P., FATEMI, SEYED MOHAMMAD REZA, & POURKAZEMI, MOHAMMAD. (2008). POPULATION GENETIC STRUCTURE OF NEOGOBIUS CASPIUS (EICHWALD, 1831) IN THE SOUTH CASPIAN SEA USING PCR-RFLP MARKER. NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES), 2(3), 21-26. SID. https://sid.ir/paper/161817/en

    Vancouver: Copy

    GHOTB RAZMJOUE E., REZVANI SOHRAB, GHAVAM MOSTAFAVI P., FATEMI SEYED MOHAMMAD REZA, POURKAZEMI MOHAMMAD. POPULATION GENETIC STRUCTURE OF NEOGOBIUS CASPIUS (EICHWALD, 1831) IN THE SOUTH CASPIAN SEA USING PCR-RFLP MARKER. NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES)[Internet]. 2008;2(3):21-26. Available from: https://sid.ir/paper/161817/en

    IEEE: Copy

    E. GHOTB RAZMJOUE, SOHRAB REZVANI, P. GHAVAM MOSTAFAVI, SEYED MOHAMMAD REZA FATEMI, and MOHAMMAD POURKAZEMI, “POPULATION GENETIC STRUCTURE OF NEOGOBIUS CASPIUS (EICHWALD, 1831) IN THE SOUTH CASPIAN SEA USING PCR-RFLP MARKER,” NEW TECHNOLOGIES IN AQUACULTURE DEVELOPMENT (JOURNAL OF FISHERIES), vol. 2, no. 3, pp. 21–26, 2008, [Online]. Available: https://sid.ir/paper/161817/en

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