GENETIC VARIATION OF BARB US CAPITO IN THE SOUTHERN CASPIAN SEA BY PCR-RFLP METHOD

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LALOUEI F.; REZVANI GILKOLAEI S.; POURKAZEMI MOHAMMAD

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Paper Information

Journal: Iranian Scientific Fisheries Journal Year:2003 | Volume:12 | Issue:1 Page(s): 117-130

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Information Journal Paper

Title

GENETIC VARIATION OF BARB US CAPITO IN THE SOUTHERN CASPIAN SEA BY PCR-RFLP METHOD

Author(s)

LALOUEI F. | REZVANI GILKOLAEI S. | POURKAZEMI MOHAMMAD | Issue Writer Certificate

Keywords

MTDNAQ3

RFLPQ3

PCRQ3

BARBUS CAPITOQ4

GENETIC VARIATIONQ4

SOUTHERN CASPIAN SEAQ3

IRANQ3

Abstract

In this study 60 samples were collected from the SOUTHERN CASPIAN SEA and some rivers of Mazandaran and Guilan provinces. GENETIC VARIATION and probable population differentiation of BARBUS CAPITO were studied based on the mitochondrial cytochrom-b gene. The mitochondrial DNA was extracted from fish fin using phenol-chlorophorm method. The specific primers were designed for B. capito and the PCR experiments were done on 60 samples. 11 restriction endonuclease enzymes were applied for RFLP analysis (ALuI, AvaI, AvaII, HinfI, DdeI, HincI, HpaII, RsaI, Sau3AI, HaeIII, TaqI). PCR products (1062 pb) and DNA digests were subjected to agarose and polyacrilamid gel electrophoresis to seperate fragments according to their molecular weight. These patterns were identified similar fpr all samples. Regarding to this patterns, it can be seen that polymorphysm phenomena cannot be observed by above mentioned enzymes and cytochrome-b gene, and there is no seperate population of B. capito in the SOUTHERN CASPIAN SEA.

Cites

References

MOLECULAR POPULATION STUDY ON PENAEUS SEMISULCATUS FROM THE PERSIAN GULF AND OMAN SEA USING CYTOCHROM OXIDASE SUBUNIT I (COL) GENE BY RFLP METHOD

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APA: Copy

LALOUEI, F., REZVANI GILKOLAEI, S., & POURKAZEMI, MOHAMMAD. (2003). GENETIC VARIATION OF BARB US CAPITO IN THE SOUTHERN CASPIAN SEA BY PCR-RFLP METHOD. IRANIAN SCIENTIFIC FISHERIES JOURNAL, 12(1), 117-130. SID. https://sid.ir/paper/5128/en

Vancouver: Copy

LALOUEI F., REZVANI GILKOLAEI S., POURKAZEMI MOHAMMAD. GENETIC VARIATION OF BARB US CAPITO IN THE SOUTHERN CASPIAN SEA BY PCR-RFLP METHOD. IRANIAN SCIENTIFIC FISHERIES JOURNAL[Internet]. 2003;12(1):117-130. Available from: https://sid.ir/paper/5128/en

IEEE: Copy

F. LALOUEI, S. REZVANI GILKOLAEI, and MOHAMMAD POURKAZEMI, “GENETIC VARIATION OF BARB US CAPITO IN THE SOUTHERN CASPIAN SEA BY PCR-RFLP METHOD,” IRANIAN SCIENTIFIC FISHERIES JOURNAL, vol. 12, no. 1, pp. 117–130, 2003, [Online]. Available: https://sid.ir/paper/5128/en

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