A COMPARATIVE STUDY ON SOME KINETIC AND BIOCHEMICAL PROPERTIES OF TRYPSIN ENZYME FROM COMMON KILKA (CLUPEONELLA CULTRIVENTRIS CASPIA) AND PORCINE

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ZAMANI ABBAS; REZAEI MASOUD; MADANI RASOUL

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Journal: Journal of Fisheries Year:2013 | Volume:66 | Issue:2 Page(s): 145-158

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Title

A COMPARATIVE STUDY ON SOME KINETIC AND BIOCHEMICAL PROPERTIES OF TRYPSIN ENZYME FROM COMMON KILKA (CLUPEONELLA CULTRIVENTRIS CASPIA) AND PORCINE

Author(s)

ZAMANI ABBAS | REZAEI MASOUD | MADANI RASOUL | Issue Writer Certificate

Keywords

COMMON KILKA (CLUPEONELLA CULTRIVENTRIS CASPIA)Q2

PORCINE TRYPSINQ2

TRYPSIN ENZYMEQ2

Abstract

In this study, the some kinetic and biochemical properties of TRYPSIN ENZYME from common kilka and porcine were assessed. The results showed that the optimum temperature and pH for common kilka trypsin and PORCINE TRYPSIN were 60oC and 8, respectively. Thermal and pH stability were also 45°C and 7-10 for common kilka trypsin and 50oC and 4-11 for PORCINE TRYPSIN. CaCI2 and MgCI2 significantly increased the trypsin activity in common kilka and porcine (P<0.05). NaCI and KCI had no significant effect on trysin activity in common kilka (P>0.05) while it was significantly decreased in porcine in presence of them (P<0.05). The trypsin activity in common kilka was significantly increased in presence of MnCI2 (P<0.05) but in porcine, showed a significant decrease (P<0.05). The comparison of inhibitors of SBTI, TLCK, PMSF and p-aminobenzamidine revealed the except for p-aminobenzamidine, the other inhibitors showed no significant difference on enzyme activity in common kilka and porcine (P>0.05) but enzyme activity was significantly decreased by p-aminobenzamidine in porcine than common kilka (P<0.05). Vmax value of common kilka trypsin was significantly higher max than that from PORCINE TRYPSIN (P<0.05). Km value of common kilka trypsin was significantly lower than that from PORCINE TRYPSIN (P<0.05). The results of this study showed that common kilka trypsin was more efficient that porcine.trypsin and it could be considered as an additive in industries and biotechnological researches in future.

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APA: Copy

ZAMANI, ABBAS, REZAEI, MASOUD, & MADANI, RASOUL. (2013). A COMPARATIVE STUDY ON SOME KINETIC AND BIOCHEMICAL PROPERTIES OF TRYPSIN ENZYME FROM COMMON KILKA (CLUPEONELLA CULTRIVENTRIS CASPIA) AND PORCINE. JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES), 66(2), 145-158. SID. https://sid.ir/paper/161975/en

Vancouver: Copy

ZAMANI ABBAS, REZAEI MASOUD, MADANI RASOUL. A COMPARATIVE STUDY ON SOME KINETIC AND BIOCHEMICAL PROPERTIES OF TRYPSIN ENZYME FROM COMMON KILKA (CLUPEONELLA CULTRIVENTRIS CASPIA) AND PORCINE. JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES)[Internet]. 2013;66(2):145-158. Available from: https://sid.ir/paper/161975/en

IEEE: Copy

ABBAS ZAMANI, MASOUD REZAEI, and RASOUL MADANI, “A COMPARATIVE STUDY ON SOME KINETIC AND BIOCHEMICAL PROPERTIES OF TRYPSIN ENZYME FROM COMMON KILKA (CLUPEONELLA CULTRIVENTRIS CASPIA) AND PORCINE,” JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES), vol. 66, no. 2, pp. 145–158, 2013, [Online]. Available: https://sid.ir/paper/161975/en

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