CLONING AND TRANSFORMATION OF IRANIAN FIREFLY LAMPYRIS TURKESTANICUS LUCIFERASE GENE (LUC) INTO TOBACCO PLANT

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

LATIF B.; JALALI JAVARAN MOKHTAR; RAJABI MEMARI H.; AZHDARI HAMED

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Plant Protection Year:2010 | Volume:32 | Issue:2 Page(s): 75-87

Download Full-Text

Persian Verion

View:

1,532

Download:

Cites:

Information Journal Paper

Title

CLONING AND TRANSFORMATION OF IRANIAN FIREFLY LAMPYRIS TURKESTANICUS LUCIFERASE GENE (LUC) INTO TOBACCO PLANT

Author(s)

LATIF B. | JALALI JAVARAN MOKHTAR | RAJABI MEMARI H. | AZHDARI HAMED | Issue Writer Certificate

Keywords

FIREFLY LUCIFERASEQ3

CLONINGQ3

GENE TRANSFORMATIONQ3

TOBACCOQ2

MOLECULAR FARMINGQ3

Abstract

“Molecular farming” is the use of plants, and potentially animals, as the means to produce therapeutic and industrial valuable proteins using genetic engineering techniques. FIREFLY LUCIFERASE is one of the most important industrial enzymes which has various applications in biotechnology and molecular biology such as microbial contamination detection by ATP amount, preparation of cancer diagnosis kits, drug screening, biosensors, protein folding and protein-protein interaction examinations, pyrosequencing, etc. luc is also used as an ideal reporter gene in order to optimize GENE TRANSFORMATION systems in genetic engineering. In this study, Iranian firefly Lampyris turkestanicus luciferase gene (luc) was cloned and introduced into TOBACCO plants. The gene was isolated by PCR and appropriate primers, designated according to luc termini, restriction sites and a plant highly expression sequence (Kozak sequence), and then cloned in pCAMBIA1304 vector, under the control of CaMV35S promoter and NOS terminator. The construct was confirmed by several methods: colony PCR, PCR, digestion, sequencing and BLAST analysis. The Agrobacterium–mediated transformation method was used to introduce luc into TOBACCO genome and putative transgenic plants were selected on the selective medium containing hygromycin and cefotaxim. Finally, transgenic plants were confirmed by PCR analysis on transgenic and control plants’ genomic DNA.

Cites

No record.

References

No record.

Cite

APA: Copy

LATIF, B., JALALI JAVARAN, MOKHTAR, RAJABI MEMARI, H., & AZHDARI, HAMED. (2010). CLONING AND TRANSFORMATION OF IRANIAN FIREFLY LAMPYRIS TURKESTANICUS LUCIFERASE GENE (LUC) INTO TOBACCO PLANT. PLANT PROTECTION (SCIENTIFIC JOURNAL OF AGRICULTURE), 32(2), 75-87. SID. https://sid.ir/paper/164916/en

Vancouver: Copy

LATIF B., JALALI JAVARAN MOKHTAR, RAJABI MEMARI H., AZHDARI HAMED. CLONING AND TRANSFORMATION OF IRANIAN FIREFLY LAMPYRIS TURKESTANICUS LUCIFERASE GENE (LUC) INTO TOBACCO PLANT. PLANT PROTECTION (SCIENTIFIC JOURNAL OF AGRICULTURE)[Internet]. 2010;32(2):75-87. Available from: https://sid.ir/paper/164916/en

IEEE: Copy

B. LATIF, MOKHTAR JALALI JAVARAN, H. RAJABI MEMARI, and HAMED AZHDARI, “CLONING AND TRANSFORMATION OF IRANIAN FIREFLY LAMPYRIS TURKESTANICUS LUCIFERASE GENE (LUC) INTO TOBACCO PLANT,” PLANT PROTECTION (SCIENTIFIC JOURNAL OF AGRICULTURE), vol. 32, no. 2, pp. 75–87, 2010, [Online]. Available: https://sid.ir/paper/164916/en

Related Journal Papers