CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF MUTATED FIREFLY LUCIFERASE FROMLAMPYRIS TURKESTANICUS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KHEIRABADI M.; GOHLKE U.; HOSSEINKHANI S.; HEINEMANN U.; NADERI MANESH H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Molecular and Cellular Researches Year:2013 | Volume:26 | Issue:2 Page(s): 174-185

Download Full-Text

Persian Verion

View:

859

Download:

Cites:

Information Journal Paper

Title

CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF MUTATED FIREFLY LUCIFERASE FROMLAMPYRIS TURKESTANICUS

Author(s)

Keywords

MUTATED (E354R/R356/H432Y) LUCIFERASEQ2

X-RAY CRYSTALLOGRAPHYQ2

LAMPYRIS TURKESTANICUSQ2

Abstract

Luciferase enzymes efficiently convert luciferin to oxyluciferin in the presence of ATP, Mg2+ and molecular oxygen. Luciferase reaction produces a spectrum with different color under various conditions. One of the most important factors is residues of structure. LAMPYRIS TURKESTANICUS, Iranian firefly, luciferase emits green light. In order to study, 3D-structure determination and red-shift mechanism analysis in comparison to other determined structure luciferase, Mutated (E354R/R356/H432Y) (emits red light) luciferase from LAMPYRIS TURKESTANICUShas been crystallized. The crystals with 2.2Å resolution of mutated firefly luciferase were obtained by the sitting drop method. The diffraction pattern from E354R/356R/H432Y luciferase belongs tetragonal system with space group P41 and unit cell dimensions a=b=85.01 and c=97.09.

Cites

No record.

References

No record.

Cite

APA: Copy

KHEIRABADI, M., GOHLKE, U., HOSSEINKHANI, S., HEINEMANN, U., & NADERI MANESH, H.. (2013). CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF MUTATED FIREFLY LUCIFERASE FROMLAMPYRIS TURKESTANICUS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 26(2), 174-185. SID. https://sid.ir/paper/248586/en

Vancouver: Copy

KHEIRABADI M., GOHLKE U., HOSSEINKHANI S., HEINEMANN U., NADERI MANESH H.. CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF MUTATED FIREFLY LUCIFERASE FROMLAMPYRIS TURKESTANICUS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2013;26(2):174-185. Available from: https://sid.ir/paper/248586/en

IEEE: Copy

M. KHEIRABADI, U. GOHLKE, S. HOSSEINKHANI, U. HEINEMANN, and H. NADERI MANESH, “CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES OF MUTATED FIREFLY LUCIFERASE FROMLAMPYRIS TURKESTANICUS,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 26, no. 2, pp. 174–185, 2013, [Online]. Available: https://sid.ir/paper/248586/en

Related Journal Papers