Analysis of the effect of effective factors in protoplast preparation of Fusarium oxysporum for using in protoplast fusion

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Rostami Afshin; SAREMI HOSSEIN

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Plant Protection Science Year:2018 | Volume:49 | Issue:1 Page(s): 155-164

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,403

Download:

Cites:

Information Journal Paper

Title

Analysis of the effect of effective factors in protoplast preparation of Fusarium oxysporum for using in protoplast fusion

Author(s)

Rostami Afshin | SAREMI HOSSEIN | Issue Writer Certificate

Keywords

Fusarium oxysporumQ1

OptimizationQ1

Osmotic stabilizerQ1

ProtoplastQ2

Abstract

Protoplast fusion as a transgenic system is an effective technique improving microorganism performances by making genetic changes. Today, exploitation of this technique has been beneficial in biocontrol of weeds. For filamentous fungi such as Fusarium species, Protoplast preparation is the first step in the common genetic transformation process. For this objective, the Fusarium oxysporum isolates were isolated from Bromrape (Orobanche sp. ) and were identified by morphological and molecular characteristics. Thereafter, the effects of various factors including medium and incubation time in spore preparation were evaluated. Furthermore, the effect of some factors on Protoplast preparation and longevity including enzyme concentrations, type and concentration of Osmotic stabilizers and lytic incubation times were also assessed. The results of this study demonstrated that Spezieller Nä hrstoffarmer Agar was the best medium to prepare spores and the best incubation time for spore germination was 16 hours. In addition, concentrations of 15mM for Glucanex and 20mM for Driselase and 5 hours incubation time were the best lytic enzyme complex and incubation time period, respectively. The KCl at the concentration of 0. 6M was the best Osmotic stabilizer in Protoplast preparation and longevity.

Cites

No record.

References

No record.

Cite

APA: Copy

Rostami, Afshin, & SAREMI, HOSSEIN. (2018). Analysis of the effect of effective factors in protoplast preparation of Fusarium oxysporum for using in protoplast fusion. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 49(1 ), 155-164. SID. https://sid.ir/paper/171682/en

Vancouver: Copy

Rostami Afshin, SAREMI HOSSEIN. Analysis of the effect of effective factors in protoplast preparation of Fusarium oxysporum for using in protoplast fusion. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2018;49(1 ):155-164. Available from: https://sid.ir/paper/171682/en

IEEE: Copy

Afshin Rostami, and HOSSEIN SAREMI, “Analysis of the effect of effective factors in protoplast preparation of Fusarium oxysporum for using in protoplast fusion,” IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 49, no. 1 , pp. 155–164, 2018, [Online]. Available: https://sid.ir/paper/171682/en

Related Journal Papers