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Information Journal Paper

Title

EVALUATION OF HYDATID CYST ANTIGENS FOR DIAGNOSIS OF HYDATIDOSIS BY DOT-ELIZA METHOD

Pages

  7-12

Abstract

 DOT-ELISA is one of the diagnostic methods for hydatidosis that has high susceptibility and known factor. In the present study, five ANTIGENs were compared by DOT-ELISA for diagnosing hydatid cyst. These ANTIGENs were homogenated protoscolex ANTIGEN (HPA), hydatid layer ANTIGEN (HLA) and ANTIGENs of fluid hydatid cyst: crude hydatid fluid ANTIGEN (CHFA), autoclaved ANTIGEN B (AAB) and boiled ANTIGEN B (BAB). TO examin every sample, first the ANTIGENs was set on the nitrocellulose membrane. Then, this membrane was placed in bovine albumin serum (BSA) %3 for blocking for an hour. After washing the membrane in PBS, it was placed in the first antibody (definite for infection) for an hour. After re-washing with PBS, it was placed in the second antibody that conjugated with peroxides. Then di-amino benzidine (DAB) substrate was used for reading the results that were recognized with color spots in places of ANTIGENs. The results show these ANTIGENs have different susceptibility and they are specific in DOT-ELISA: CHFA (54.8% &39.1%), BAB (54.8% &56.5%), AAB (45.2% & 67.4%), HPA (78.6% &30.4%) and HLA (57.1% &50%). Protoscolex ANTIGEN has the highest susceptibility and AAB has the highest attributes. To increasing accuracy in hydatid diagnosis, it is better to test serum samples with a high susceptibility test and then a test may be applied with the higher susceptibility.

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    Cite

    APA: Copy

    HOUSHMAND, E., & DADASH BEYGI, M.. (2009). EVALUATION OF HYDATID CYST ANTIGENS FOR DIAGNOSIS OF HYDATIDOSIS BY DOT-ELIZA METHOD. JOURNAL OF LARGE ANIMAL CLINICAL SCIENCE RESEARCH (JOURNAL OF VETERINARY MEDICINE), 3(6), 7-12. SID. https://sid.ir/paper/175495/en

    Vancouver: Copy

    HOUSHMAND E., DADASH BEYGI M.. EVALUATION OF HYDATID CYST ANTIGENS FOR DIAGNOSIS OF HYDATIDOSIS BY DOT-ELIZA METHOD. JOURNAL OF LARGE ANIMAL CLINICAL SCIENCE RESEARCH (JOURNAL OF VETERINARY MEDICINE)[Internet]. 2009;3(6):7-12. Available from: https://sid.ir/paper/175495/en

    IEEE: Copy

    E. HOUSHMAND, and M. DADASH BEYGI, “EVALUATION OF HYDATID CYST ANTIGENS FOR DIAGNOSIS OF HYDATIDOSIS BY DOT-ELIZA METHOD,” JOURNAL OF LARGE ANIMAL CLINICAL SCIENCE RESEARCH (JOURNAL OF VETERINARY MEDICINE), vol. 3, no. 6, pp. 7–12, 2009, [Online]. Available: https://sid.ir/paper/175495/en

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