JOURNAL OF LARGE ANIMAL CLINICAL SCIENCE RESEARCH (JOURNAL OF VETERINARY MEDICINE)
Year:2009 | Volume:3 | Issue:6|Papers Count:13

The main aim of this research is to study nuclear localization of mouse PPARg1 cDNA into pEGFP-C1. To investigate the nuclear localization of PPARg1 protein linked to EGFP marker gene into bovine fibroblast and analysis of intracellular green fluorescency Mouse PPARg1 cDNA in a mammalian expression vector (pEGFP-C1) in a chimeric cDNA type, encompassing PPARg1 with EGFP cDNA. EGFP marker was used for monitoring. To confirm the intracellular localization of EGFP- PPARg1, bovine fibroblast cells were transfected with the 2.4 mg constructed plasmid and 6 ml Lipofectamine 2000. The related product was entered into the nucleus of bovine fibroblasts after transfection of its cDNA. As expected, chimeric cDNA of EGFP-PPARg1 correctly expressed and related protein was dominantly entered to the nuclei of the cells. Thus the recombinant plasmid could be applicable for further studies to unravel the further aspects of PPARg1 function. Moreover fusion of EGFP with PPARg1 does not hamper the nuclear targeting of PPARg1.

Dot-ELISA is one of the diagnostic methods for hydatidosis that has high susceptibility and known factor. In the present study, five antigens were compared by dot-ELISA for diagnosing hydatid cyst. These antigens were homogenated protoscolex antigen (HPA), hydatid layer antigen (HLA) and antigens of fluid hydatid cyst: crude hydatid fluid antigen (CHFA), autoclaved antigen B (AAB) and boiled antigen B (BAB). TO examin every sample, first the antigens was set on the nitrocellulose membrane. Then, this membrane was placed in bovine albumin serum (BSA) %3 for blocking for an hour. After washing the membrane in PBS, it was placed in the first antibody (definite for infection) for an hour. After re-washing with PBS, it was placed in the second antibody that conjugated with peroxides. Then di-amino benzidine (DAB) substrate was used for reading the results that were recognized with color spots in places of antigens. The results show these antigens have different susceptibility and they are specific in dot-ELISA: CHFA (54.8% &39.1%), BAB (54.8% &56.5%), AAB (45.2% & 67.4%), HPA (78.6% &30.4%) and HLA (57.1% &50%). Protoscolex antigen has the highest susceptibility and AAB has the highest attributes. To increasing accuracy in hydatid diagnosis, it is better to test serum samples with a high susceptibility test and then a test may be applied with the higher susceptibility.

In present study, we determined the effects of doxycycline on the serum biochemical parameters of Broilers. Forty Broilers (19 days old) were randomly divided into two groups of control and treatment. Bath groups were kept under the same environmental condition and nutrition. In the treatment group, 400 gr/ 1000 lit doxycycline was administered. After five days blood samples of all birds were collected. And their sera separated by centrifuging, then some of biochemical parameters were evaluated. Results showed that Alkaline phosphatase (ALP), Alanine aminotransferas (ALT), Aspartate aminotransferase (AST), billirubin and billiverdin concentration were significantly increased (p<0.05), but Calcium concentration was significantly decreased in treatment group. Evaluation of the serum levels of glucose and Phosphorous did not reveal statistically significant differences between treatment and control groups. In assessment of results, doxycycline administration can be used in broilers but it must be controlled in liver diseases because it can cause changes on liver parameters.

In addition to sanitary importance to supply human food, parasite infections may cause a lot of economic damages to animal population of a specific region, that is because of insufficient growth and deletion or clear up of infected carcass. The aim of this study is to survey of cestoda infection in digestive system of slaughtered sheep and goat in Baneh slaughter house in order to do necessary measurements by showing the optimal solutions to decrease this kind of parasitism infection by considering the rate of its outbreak percentage. Samples were collected from 186 slaughtered sheep and 252 goats in a year at Baneh slaughter house, and they are sent to Orumia Islamic Azad university lab to be painted. Then complementary studies were conducted to determine the cestoda. The results illustrated 6 effected M.benideni and 4 M. expansa among sheep and 1 M. benideni and 2 M. expansa among goats. The percent aye of whole infectious among sheep was 5.37% while there was only 1.19% among goats.

Bodjagh wetland, which is about 80 hectare, is one of the conserved and important wetlands in Guilan. No study was done to identify fish parasites in this important wetland yet. This study was conducted in order to identify the parasites of two commercial important fishes (pike & roach) of this wetland. Parasite infection of 36 specimens of roach (Rutilus rutilus caspicus) and 39 specimens of pike (Esox lusius) were caught in Bodjagh wetland in the years 2004-2005 by drift net, sein net and cast net were studied. The fish were transported to the laboratory alive and their skin, muscles, digestion tract, gills and eyes were studied carefully by microscope and stereoscope. According to the  results, 6 species  of parasites  recognized  in the both fishes, including: Raphidascaris acus, Diplostomum spathaceum and Tetraonchus monenteron in pike (Esox lusius) and  Caryophyllaceous fimbriceps, Diplostomum spathaceum,  Piscicola sp. And Lernaea sp. In roach (Rutilus rutilus caspicus). Three species of C. fimbriceps, Piscicola sp. and Lernaea sp. are reported in roacth for the first time in Iran. Totally the amount of affliction to parasites in roach was more than pike. Both of the fishes had parasites in digestion tract, gill and eye. The parasites in digestion tract and gill were different. The most occurrences of parasites were related to Diplostomum spathaceum because of the living habitat of these fishes (wetland). 

Propofol is a short-acting intravenous general anesthetic for induction and maintenance of general anesthesia. Propofol used alone or in combination with other CNS depressant drugs in human and animals. Nowadays studies considering usage of anesthetic drugs in poultries for research and surgery purpose are in developing. Injectable drugs have problems in poultry, so inhalant anesthetic drugs are preferred. But sometimes inhalant anesthetic drugs have problems too. In this experimental study, we evaluate serum biochemical change subsequent propofol-induced general anesthesia in adult aborigine hen. Therefore 10 adult aborigine hens selected randomly. To induce anesthesia propofol (7.5mg/kg) injected intravenously and for maintenance propofol (0.5mg/kg/min) infused until 90 minute with infusion pump. During anesthesia the following biochemical factors were determined in 0, 15, 30, 60, 90 minutes and 24, 72 hours after anesthesia. Serum albumin, alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphates (ALP), bilirubin, blood urea nitrogen (BUN) and creatinine concentration were measured. Results showed that: bilirubin, BUN, ALP and creatinine concentrations significantly changed (p<0.05), but ALT, AST and albumin concentration had none significantly changed. Using this drug in poultry’s anesthesia and surgery needs to be investigated more.

This experiment was carried out to determine the effects of different levels of protein and electrolyte balance (DEB) on performance, biochemical and haematological parameters in Ross (308) broiler exposed to heat stress. In this experiment, 486, one-day old broilers were used in a completely randomized design with a 3´3 factorial arrangement with 3 replicate for a treatment. The diets were formulated according to NRC (1994) recommendation with protein levels (100, 90 and 80% of NRC recommendations) and electrolyte balance (200, 260 and 320 meq/kg). The birds were exposed to heat stress (34±30C) for 8 hours/day (10:00 to 18:00). The results of experiment indicated that body weight gain was significantly different in starter and in broilers fed containing 260 meq/kg DEB and protein (90%) were significantly higher than those fed other levels of DEB and protein, in all periods (starter, grower and total period). In comparison to 200 DEB, the level of 260 DEB increased body weight gain and feed intake in all periods (p<0.05). Dietary protein levels had no significant effect on body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR). Dietary DEB and protein had no significant effect on cholesterol, triglyceride, total protein, albumin, sodium and potassium. The experimental groups did not affect on Haemoglobolin, Haematocrit, hetrophyl, lymphocyte, monocyt and White blood cells counts. The concentration of chlorine was decreased by increasing DEB (p<0.05). As a result, using of DEB 260 meq/kg and medium protein level (90% NRC) for broilers exposed to heat stress is recommended.

The aim of this research was to study the prevalence of Cryptosporidium in human and some animals that have close relationship with human. In order, 100 samples of feces referred to Parasitology department of central laboratory of East Azerbaijan province (Tabriz city) were studied with light microscopy after staining with Modified Ziehl-Nelson method and then 100 feces samples of calves around Tabriz were collected, and at last the feces samples collected from 50 wild rats from different parts of Tabriz and were studied. Result of this study was showed that prevalence of infestation was 12%, 16% and 74% in humans, calves and rat respectively. According to these results, the infestation was high in three studied group (human, calves and rats) and it can be related with its wide distribution and it’s a lot of hosts. Cryptosporidium oocysts are reason for disease transmission between human and animals. Regarding that medical and veterinarian laboratories usually don’t care about Cryptosporidium in diarrhea cases, there is possibility of increase in Cryptosporidium prevalence and incidence in future.