IDENTIFICATION OF DIFFERENT SYMBIOSIS FUNGUS IN ORDER TO REPRODUCE AND DEVELOPMENT

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AJORLOU Z.; FOROUHI FATEMEH

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Journal: JOURNAL OF CROP ECOPHYSIOLOGY Year:2011 | Volume:3 | Issue:2 Page(s): 170-181

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Title

IDENTIFICATION OF DIFFERENT SYMBIOSIS FUNGUS IN ORDER TO REPRODUCE AND DEVELOPMENT

Author(s)

AJORLOU Z. | FOROUHI FATEMEH | Issue Writer Certificate

Keywords

IDENTIFICATIONQ2

SYMBIOSIS FUNGUSQ3

REPRODUCE AND DEVELOPMENTQ2

Abstract

In order to study the IDENTIFICATION of different SYMBIOSIS FUNGUS in order to REPRODUCE AND DEVELOPMENT, this experiment was conducted at Iran in 2011. First selected rhizosphere soil of weed from experimental field at Shahr-e-Qods zone in Tehran and for removing fungal spores was used suspension cultured plant by water and passing the sieve 60, 100 and 400 mesh. Materials collected on 400 mesh sieve were transferred 60% sucrose and was centrifuged for three minutes at 2700 rpm. The upper suspension of centrifuge was transferred 400 mesh sieve and was washed by distilled water. The part of the material collected on 400 mesh sieve was transferred Petri dish and was removed spores with non-active hyphae with view sample by Binocular and pipette eppendorf and then was transferred tube 1.5×6 cm containing 2 ml of distilled water. The tubes were placed on ice and fungal spores community was visible by naked eye bottom of the tubes. To determine the number of active hyphae was used Tetrazolium standard method. Thus the number of spores in 10 gr of soil was placed on 1% MTT solution at 35°C for 24h. Then active hyphae and non-active hyphae were seen red and no-color after placed spores under a microscope respectively. The active hyphae was determined by counting the number of color spores in 10 g of soil. After isolation and study of spores 5 fungi were identified with following names: Glomus mosseae, Glomus intraradices, Glomus caledonium, Glomus clarum and Glomus etunicatum and Glomus mosseae was the dominant with a frequency of 86%.

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APA: Copy

AJORLOU, Z., & FOROUHI, FATEMEH. (2011). IDENTIFICATION OF DIFFERENT SYMBIOSIS FUNGUS IN ORDER TO REPRODUCE AND DEVELOPMENT. JOURNAL OF CROP ECOPHYSIOLOGY, 3(2), 170-181. SID. https://sid.ir/paper/176433/en

Vancouver: Copy

AJORLOU Z., FOROUHI FATEMEH. IDENTIFICATION OF DIFFERENT SYMBIOSIS FUNGUS IN ORDER TO REPRODUCE AND DEVELOPMENT. JOURNAL OF CROP ECOPHYSIOLOGY[Internet]. 2011;3(2):170-181. Available from: https://sid.ir/paper/176433/en

IEEE: Copy

Z. AJORLOU, and FATEMEH FOROUHI, “IDENTIFICATION OF DIFFERENT SYMBIOSIS FUNGUS IN ORDER TO REPRODUCE AND DEVELOPMENT,” JOURNAL OF CROP ECOPHYSIOLOGY, vol. 3, no. 2, pp. 170–181, 2011, [Online]. Available: https://sid.ir/paper/176433/en

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