REGENERATION AND TRANSFORMATION OF HAPLOID LEAF AND EMBRYOGENIC TISSUES DERIVED FROM OVULE CULTURE IN SUGAR BEET (BETA VULGARIS L.)

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NOROUZI P.

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ISSN: 2588-4824

Journal Paper

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Journal: JOURNAL OF CROP BREEDING Year:2013 | Volume:4 | Issue:10 Page(s): 63-79

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Title

REGENERATION AND TRANSFORMATION OF HAPLOID LEAF AND EMBRYOGENIC TISSUES DERIVED FROM OVULE CULTURE IN SUGAR BEET (BETA VULGARIS L.)

Author(s)

NOROUZI P. | Issue Writer Certificate

Keywords

HAPLOIDQ3

EMBRYOQ2

AGROBACTERIUMQ2

SUGAR BEETQ3

TRANSFORMATIONQ2

Abstract

In order to use of HAPLOID tissue of SUGAR BEET for producing transgenic plants, at first, some experiments were accomplished for shoot production of HAPLOID leaves from HAPLOID EMBRYOs. Results of current study indicated that MSB or PGoB medium supplemented with 3% sucrose, 0.2 mgl-1 BA and 0.5 mgl-1 NAA could be used for increasing HAPLOID EMBRYOs and half strength MSB medium contains 1% sucrose to develop EMBRYOs and shoot production. Moreover, PGoB medium supplemented with 1 mgl-1 BA and 1 mgl-1 NAA was used for shoot production from HAPLOID leaves. In the next step, HAPLOID EMBRYOgenic calli and leaves were inoculated with AGROBACTERIUM tumefaciens cells harboring pBI121 binary vector. After 2 days co-culture, the inoculated explants were transferred to selection medium contains 50 mgl-1 kanamycin. After 3-5 weeks, putative transgenic shoots were analyzed by GUS staining. Results showed that two explants have ability for shoot regeneration of HAPLOID transgenic cells.

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APA: Copy

NOROUZI, P.. (2013). REGENERATION AND TRANSFORMATION OF HAPLOID LEAF AND EMBRYOGENIC TISSUES DERIVED FROM OVULE CULTURE IN SUGAR BEET (BETA VULGARIS L.). JOURNAL OF CROP BREEDING, 4(10), 63-79. SID. https://sid.ir/paper/181193/en

Vancouver: Copy

NOROUZI P.. REGENERATION AND TRANSFORMATION OF HAPLOID LEAF AND EMBRYOGENIC TISSUES DERIVED FROM OVULE CULTURE IN SUGAR BEET (BETA VULGARIS L.). JOURNAL OF CROP BREEDING[Internet]. 2013;4(10):63-79. Available from: https://sid.ir/paper/181193/en

IEEE: Copy

P. NOROUZI, “REGENERATION AND TRANSFORMATION OF HAPLOID LEAF AND EMBRYOGENIC TISSUES DERIVED FROM OVULE CULTURE IN SUGAR BEET (BETA VULGARIS L.),” JOURNAL OF CROP BREEDING, vol. 4, no. 10, pp. 63–79, 2013, [Online]. Available: https://sid.ir/paper/181193/en

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