Effects of Different Media Factors on In Vitro Proliferation of St. John’ s Wort (Hypericum perforatum)

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Parsamanesh Zeynab; HEDAYAT MOHAMMAD; BAYAT FERESHTEH

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ISSN: 2588-4824

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Journal: JOURNAL OF CROP BREEDING Year:2018 | Volume:10 | Issue:27 Page(s): 75-83

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Title

Effects of Different Media Factors on In Vitro Proliferation of St. John’ s Wort (Hypericum perforatum)

Author(s)

Parsamanesh Zeynab | HEDAYAT MOHAMMAD | BAYAT FERESHTEH | Issue Writer Certificate

Keywords

BAQ2

Hypericum perforatumQ2

ExplantQ2

ProliferationQ2

SucroseQ3

St.John’s wortQ3

Abstract

St. John’ s wort is a traditional medicinal plant that has been used from ancient time for the treatment of neurological and psychiatric diseases. A successful in vitro propagation system for the St. John’ s wort was optimized. Different Explant type (single node and stem cutting with two nodes) were used in Murashige and Skoog (MS) medium in different concentrations (MS full, 3/4 MS and 2/3 MS) supplemented with BA (0. 05, 1. 0, 2. 0 mgl-1) and Sucrose (30 and 45 gl-1). The optimum Proliferation rate of St. John’ s wort was obtained in 2/3 MS medium containing 30 gl-1 of Sucrose and 0. 1mgl-1 BA with single node Explant. Also, evaluation of results revealed that the number of proliferated shoots will increased in MS media supplemented with BA along with reduced concentrations of Sucrose. The in vitro generated shoots were cultured on half-strength MS medium containing 0, 0. 2, 0. 4, 0. 6, 0. 8 and 1 mgl-1 butyric acid (IBA). The results showed that all proliferated shoot were rooted in plant growth regulators free medium. But, using and increasing the concentration IBA in rooting media can increases the number of roots and average of root length per each Explant.

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APA: Copy

Parsamanesh, Zeynab, HEDAYAT, MOHAMMAD, & BAYAT, FERESHTEH. (2018). Effects of Different Media Factors on In Vitro Proliferation of St. John’ s Wort (Hypericum perforatum). JOURNAL OF CROP BREEDING, 10(27 ), 75-83. SID. https://sid.ir/paper/181204/en

Vancouver: Copy

Parsamanesh Zeynab, HEDAYAT MOHAMMAD, BAYAT FERESHTEH. Effects of Different Media Factors on In Vitro Proliferation of St. John’ s Wort (Hypericum perforatum). JOURNAL OF CROP BREEDING[Internet]. 2018;10(27 ):75-83. Available from: https://sid.ir/paper/181204/en

IEEE: Copy

Zeynab Parsamanesh, MOHAMMAD HEDAYAT, and FERESHTEH BAYAT, “Effects of Different Media Factors on In Vitro Proliferation of St. John’ s Wort (Hypericum perforatum),” JOURNAL OF CROP BREEDING, vol. 10, no. 27 , pp. 75–83, 2018, [Online]. Available: https://sid.ir/paper/181204/en

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