MOLECULAR INVESTIGATION OF BLAD DISORDER IN BREEDER BULLS OF WEST AND NORTH-WEST ANIMAL BREEDING CENTER

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PIRAHARY O.; NOOMI S.; BADAMI M.R.; ELYASI ZARRINGHABAIE GH.; GHAFFARY I.

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Journal: JOURNAL OF CROP ECOPHYSIOLOGY (AGRICULTURE SCIENCE) Year:2009 | Volume:3 | Issue:11 Page(s): 39-46

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Title

MOLECULAR INVESTIGATION OF BLAD DISORDER IN BREEDER BULLS OF WEST AND NORTH-WEST ANIMAL BREEDING CENTER

Author(s)

Keywords

BLADQ3

HOLSTEINQ3

PCR-RFLPQ3

SEMEN AND TAQI ENZYMEQ3

Abstract

Autosomal recessive genetic diseases in cattle are breed-specific and one of them is bovine leukocyte adhesion deficiency syndrome (BLAD). It is a genetic disease that affects especially the HOLSTEIN breed. The molecular basis of BLAD is a single point mutation (adenine to guanine) at position 383 of the CD18 gene, which caused an aspartic acid to glycine substitution at amino acid 128 (D128G) in the adhesion molecule CD18. Recognition of these disorders presently was carried out by PCRRFLP with TaqI and HaeIII enzymes. In this research, DNA samples purified from bulls and then investigated the quality and quantity of extracted DNA.101bp of CD18 gene amplificated and electrophoresis carried out on 2% agaros gel. Digested products run on 10% polyacrylamide gel and then stained by etidium bromide. None of the bulls under study were found to be BLAD carrier and all recognized as TL code.

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APA: Copy

PIRAHARY, O., NOOMI, S., BADAMI, M.R., ELYASI ZARRINGHABAIE, GH., & GHAFFARY, I.. (2009). MOLECULAR INVESTIGATION OF BLAD DISORDER IN BREEDER BULLS OF WEST AND NORTH-WEST ANIMAL BREEDING CENTER. JOURNAL OF CROP ECOPHYSIOLOGY (AGRICULTURE SCIENCE), 3(11), 39-46. SID. https://sid.ir/paper/182918/en

Vancouver: Copy

PIRAHARY O., NOOMI S., BADAMI M.R., ELYASI ZARRINGHABAIE GH., GHAFFARY I.. MOLECULAR INVESTIGATION OF BLAD DISORDER IN BREEDER BULLS OF WEST AND NORTH-WEST ANIMAL BREEDING CENTER. JOURNAL OF CROP ECOPHYSIOLOGY (AGRICULTURE SCIENCE)[Internet]. 2009;3(11):39-46. Available from: https://sid.ir/paper/182918/en

IEEE: Copy

O. PIRAHARY, S. NOOMI, M.R. BADAMI, GH. ELYASI ZARRINGHABAIE, and I. GHAFFARY, “MOLECULAR INVESTIGATION OF BLAD DISORDER IN BREEDER BULLS OF WEST AND NORTH-WEST ANIMAL BREEDING CENTER,” JOURNAL OF CROP ECOPHYSIOLOGY (AGRICULTURE SCIENCE), vol. 3, no. 11, pp. 39–46, 2009, [Online]. Available: https://sid.ir/paper/182918/en

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